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Catalog Number	Size	Price
LS-C661867-10	10 µg	$318
LS-C661867-100	100 µg	$470

ABCB11 / BSEP Antibody - Immunohistochemistry - Anti-ABCB11/Bsep Picoband Antibody

ABCB11 / BSEP Antibody - IF analysis of ABCB11 using anti-ABCB11 antibody ABCB11 was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-ABCB11 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

ABCB11 / BSEP Antibody - Western blot - Anti-ABCB11/Bsep Picoband Antibody

ABCB11 / BSEP Antibody - Flow Cytometry analysis of A431 cells using anti-ABCB11 antibody. Overlay histogram showing A431 cells stained with anti-ABCB11 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABCB11 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Polyclonal Rabbit anti‑Human ABCB11 / BSEP Antibody (IHC, WB) LS‑C661867

Name: Anti-ABCB11 / BSEP Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C661867-10
Price: 318.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

ABCB11 / BSEP Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IHC-P, ICC, WB, Flo

Reactivity:

Human

Format:

Unconjugated, Unmodified

$318

LS-C661867-10

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

ABCB11 / BSEP Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IHC-P, ICC, WB, Flo

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

BSEP antibody LS-C661867 is an unconjugated rabbit polyclonal antibody to human BSEP (ABCB11). Validated for Flow, ICC, IHC and WB.

Target

Human ABCB11 / BSEP

Synonyms

See All ABCB11 / BSEP Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

A synthetic peptide corresponding to a sequence at the C-Terminus of human ABCB11 (1175-1199 aa KYGDNTKEIPMERVIAAAKQAQLHD), different from the related mouse and rat sequences by three amino acids.

Specificity

Expressed predominantly, if not exclusively in the liver, where it was further localized to the canalicular microvilli and to subcanalicular vesicles of the hepatocytes by in situ.

Applications

IHC
IHC - Paraffin
ICC
Western blot
Flow Cytometry

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

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About ABCB11 / BSEP

Uniprot: O95342 NCBI: NM_003742 NP_003733.2