Rat IgG1 + IgG2a + IgG2b + IgG2c Fc

Goat

Rat (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated. Also available conjugated with FITC, Biotin, HRP, TRITC.

Purified IgG

Unmodified

Purified normal IgG, including all subclasses, isolated from pooled rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Rat IgG, Fc specific.

• Immunofluorescence
• ELISA
• Dot Blot

The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule including all subclasses. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against normal rat plasma and serum, a characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG. No reaction is obtained with purified IgA, IgM and IgG/Fab fragments or any other serum protein. As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Typical working dilutions in histochemistry are usually between 1:50 and 1:250. in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5000.

Lyophilized from PBS, pH 7.2, No preservatives added

Reconstitute with distilled water.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.