Rat IgG1 + IgG2a + IgG2b + IgG2c Fc

Goat

Rat (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated. Also available conjugated with FITC, Biotin, HRP, TRITC.

Antiserum

Unmodified

Purified normal IgG, including all known subclasses, isolated from pooled rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Rat IgG, Fc specific.

• Immunodiffusion

The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule including all subclasses. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against normal rat plasma and serum, a characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG. No reaction is obtained with purified IgA, IgM and IgG/Fab fragments or any other serum protein. In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG. as secondary antibody to precipitate the immunoglobulin in normal rat serum. to prepare an immunoadsorbent for the production of Ig-free rat serum, plasma or non-Ig preparations.

Delipidated, heat-inactivated, lyophilized antiserum. No preservative.

1 ml Distilled water

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.