Target
Human IGF2
Synonyms
IGF2 | C11orf43 | IGF-II | PP9974 | Somatomedin-A
Reactivity
Human
(tested or 100% immunogen sequence identity)
Purification
Ion exchange chromatography
Immunogen
Highly purified recombinant human IGF-II.
Specificity
Recognizes Insulin-Like Growth Factor II (IGF-II).
Applications
- Western blot (0.1 - 0.2 µg/ml)
- ELISA
- Functional Assay
- Neutralization
Usage
Suitable for use in ELISA, Neutralization and Western Blot. Direct ELISA: < 0.5 ug detects 0.2-0.4 ng/well of recombinant hIGF-II using 100 ul/well antibody solution. Neutralization: 3.1-4.6 mg/ml yields one-half maximal inhibition [ND50] of the biological activity of hIGF-II (10 ng/ml). Western Blot: 0.1-0.2 ug/ml detects recombinant hIGF-II at 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.2, 0.1% Sodium Azide
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee