The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect Rat IFN-gamma by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IFN-gamma. To detect Rat IFN-gamma by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Rat IFN-gamma (
LS-C104611) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IFN-gamma. Western Blot: To detect Rat IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IFN-gamma is 1.5-3 ng/lane, under either reducing or non-reducing conditions.