Target
Human IDUA / MPS1
Synonyms
IDUA | Alpha-L-iduronidase | MPS1 | Iduronidase, alpha-L- | IDA
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
NS0-derived rhIDUA. (aa 26 - 653).
Specificity
Recognizes human IDUA.
Applications
- Western blot (0.1 - 0.2 µg/ml)
- Immunoprecipitation
- ELISA (0.5 - 1 µg/ml)
Usage
Suitable for use in Direct ELISA, Western Blot and Immunoprecipitation. Direct ELISA: 0.5-1.0 ug/ml; Detection limit for rhIDUA is ~0.2 ng/well. Western Blot: 0.1-0.2 ug/ml; Detection limit for rhIDUA is ~5 ng/lane under non-reducing and reducing conditions. Antibody tested in WB and ELISA using the full-length recombinant protein. Immunoprecipitation: This antibody has been used to Immunoprecipitation rhIDUA from conditioned media of transfected NSO cells.
Presentation
Lyophilized from PBS, pH 7.4, 5% Trehalose
Reconstitution
Reconstitute with 500uL of sterile PBS.
Storage
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee