Target
Human IDUA / MPS1
Synonyms
IDUA | Alpha-L-iduronidase | MPS1 | Iduronidase, alpha-L- | IDA
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
NS0-derived, recombinant human IDUA aa26-653.
Specificity
Recognizes human Alpha-L-Iduronidase. Species sequence Homology: Mouse (79%), chicken (61%).
Applications
- Western blot (1 - 2 µg/ml)
- ELISA (0.5 - 1 µg/ml)
Usage
Suitable for use in ELISA and Western Blot. Direct ELISA: 0.5-1 ug/ml. The detection limit is ~1 ng/well. Antibody was tested using full-length, recombinant protein. Western Blot: 1-2 ug/ml. Using a colorimetric detection system, the detection limit is ~5 ng/lane under non-reducing and reducing conditions. Antibody tested in WB using the full-length recombinant protein. Chemiluminescent detection will increase sensitivity by 5-50 fold.
Presentation
Lyophilized from PBS, pH 7.4, 5% Trehalose
Reconstitution
Reconstitute in 200 ul sterile PBS
Storage
Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 1 year at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee