Human IDS / Iduronate 2 Sulfatase

Goat

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

NS0-derived rhIDS (aa26-550).

aa26-550

Selected for its ability to recognize human IDS in direct ELISAs and Western Blots. In these formats, this antibody shows ~50% cross-reactivity with rmIDS.

• Western blot
• Immunoprecipitation
• ELISA

Western Blot: This antibody can be used at 0.1-0.2 ug/mL with the appropriate secondary reagents to detect human IDS. The detection limit for rhIDS is approximately 25 ng/lane under non-reducing and reducing conditions. Immunoprecipitation: This antibody has been used to immunoprecipitate rhIDS from conditioned media of transfected NS0 cells. The recovered rhIDS can be detected by Western Blot analysis. Direct ELISA: This antibody can be used at 0.5-1.0 ug/mL with the appropriate secondary reagents to detect human IDS. The detection limit for rhIDS is approximately 0.3 ng/well.

Lyophilized from PBS, pH 7.4, 5% Trehalose

Reconstitute with 500uL of sterile PBS.

Store at -20°C. After reconstitution, aliquot and store at -20°C. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P22304 NCBI: NM_000202 NP_000193.1