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Catalog Number	Size	Price
LS-C344975-100	100 µg	$424

IDO1 / IDO Antibody - Immunocytochemistry fluorescent staining of human mature dendritic cells using anti-IDO (human), pAb .

IDO1 / IDO Antibody - Western blot analysis of human IDO using anti-IDO (human), pAb at 1:2,000 dilution.1. Recombinant human IDO (His tagged).2. PHA- treated human peripheral blood lymphocyte lysate.

IDO1 / IDO Antibody - LPS induction of IDO in THP-1 cells: To examine the ability of induction of IDO with LPS, reactions were carried out at 37 degrees C over 8, 16, 20, 24, 32, 40, 48hrs, respectively by adding LPS (1 ug/ml) to THP-1 cells, which were not maintained with serum starvation. LPS in lanes 2, 3, 4, 5, 6, 7, and 8 were subjected to LPS-responsive THP-1 cell treatments over 8, 16, 20, 24, 32, 40, 48hrs, respectively. Lane 1 was not treated with LPS. IDO was detected by using anti-IDO (human), pAb .

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Polyclonal Rabbit anti‑Human IDO1 / IDO Antibody (IF, WB) LS‑C344975

Name: Anti-IDO1 / IDO Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C344975-100
Price: 424.0000 USD
Availability: InStock

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Antibody:

IDO1 / IDO Rabbit anti-Human Polyclonal Antibody

Application:

ICC, IF, WB, Flo, ELISA, Func

Reactivity:

Human

Format:

Unconjugated, Unmodified

$424

LS-C344975-100

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

IDO1 / IDO Rabbit anti-Human Polyclonal Antibody

Application:

ICC, IF, WB, Flo, ELISA, Func

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

IDO antibody LS-C344975 is an unconjugated rabbit polyclonal antibody to human IDO (IDO1). Validated for ELISA, Flow, Func, ICC, IF and WB.

Target

Human IDO1 / IDO

Synonyms

See All IDO1 / IDO Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Protein A purified

Modifications

Unmodified

Immunogen

Recombinant human IDO.

Specificity

Recognizes human IDO. Detects a band of ~45kDa by Western blot.

Applications

ICC (1:500)
Immunofluorescence
Western blot (1:2000 - 1:5000)
Flow Cytometry (1:200 - 1:500)
ELISA (1:2000 - 1:10000)
Functional Assay

Usage

ELISA - Direct and indirect: 1:2000-1:10000. Flow Cytometry - 1:200-1:500. Immunocytochemistry - 1:500. Western Blot - 1:2000-1:5000 using ECL. suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. suggested incubation time is 1 hour at room temperature.

Presentation

PBS, pH 7.4, no preservatives.

Storage

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About IDO1 / IDO

Uniprot: P14902 NCBI: NM_002164 AAA36081.1