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Catalog Number	Size	Price
LS-C153780-100	100 µg (1.09 mg/ml)	$574

IDO1 / IDO Antibody - Western blot of Mouse Anti-IDO1 Antibody. Lane 1: untreated HeLa cells. Lane 2: IFN-r treated HeLa cells. Load: 35 ug per lane. Primary antibody: IDO 1 Antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800 mouse secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 41-42 kDa, 41-42 kDa for IDO-1. Other band(s): none.

Monoclonal Mouse anti‑Human IDO1 / IDO Antibody (clone 10.1, IHC, IF, WB) LS‑C153780

Name: Anti-IDO1 / IDO Antibody | Mouse anti-Human Monoclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C153780-100
Price: 574.0000 USD
Availability: InStock
Rating: 4.4 (1 reviews)

(4.4) Rating

Submit Review (5) Publication(s) LSBio Guarantee

Note: This antibody replaces LS-C45686

Antibody:

IDO1 / IDO Mouse anti-Human Monoclonal (10.1) Antibody

Application:

IHC, IF, WB, IP, ELISA

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

$574

LS-C153780-100

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

IDO1 / IDO Mouse anti-Human Monoclonal (10.1) Antibody

Application:

IHC, IF, WB, IP, ELISA

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

Specifications

Description

IDO antibody LS-C153780 is an unconjugated mouse monoclonal antibody to IDO (IDO1) from human. It is reactive with human and mouse. Validated for ELISA, IF, IHC, IP and WB. Cited in 5 publications.

(5) Publication(s)

Target

Human IDO1 / IDO

Synonyms

See All IDO1 / IDO Antibodies

Host

Mouse

Reactivity

Human, Mouse (tested or 100% immunogen sequence identity)

Clonality

IgG3 Monoclonal

Clone

10.1

Conjugations

Unconjugated

Purification

Protein G affinity chromatography

Modifications

Unmodified

Immunogen

Anti-IDO1 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with fragment of recombinant human and mouse IDO1 protein followed by hybridoma development.

Specificity

This antibody is specific for human and mouse IDO1 protein. A BLAST analysis was used to suggest cross-reactivity with IDO1 from human and mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with IDO1 from other sources has not been determined.

Applications

IHC (1:100 - 1:500)
Immunofluorescence (1:50 - 1:100)
Western blot (1:500 - 1:1500)
Immunoprecipitation
ELISA (1:5000 - 1:50000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Anti-IDO1 antibody has been tested for use in ELISA, Western Blot, IP and IHC. Specific conditions for reactivity should be optimized by the end user.

Presentation

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Storage

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About IDO1 / IDO

Uniprot: P14902 NCBI: NM_002164 AAA36081.1

LSBio Ratings

IDO1 / IDO Antibody (clone 10.1) for IHC, IF/Immunofluorescence, WB/Western, IP, ELISA LS-C153780 has an LSBio Rating of

(4.4) Rating

Publications (4.4)

Learn more about The LSBio Ratings Algorithm

Publications (5)

Synovial autoreactive T cells in rheumatoid arthritis resist IDO-mediated inhibition. Zhu L, Ji F, Wang Y, Zhang Y, Liu Q, Zhang JZ, Matsushima K, Cao Q, Zhang Y. Journal of immunology (Baltimore, Md. : 1950). 2006 177:8226-33.

PubMed: 17114500

Imaging correlates of differential expression of indoleamine 2,3-dioxygenase in human brain tumors. Batista CE, Juhsz C, Muzik O, Kupsky WJ, Barger G, Chugani HT, Mittal S, Sood S, Chakraborty PK, Chugani DC. Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging. 11:460-6.

PubMed: 19434461 PMC: PMC2763988

Psoriasis is characterized by deficient negative immune regulation compared to transient delayed-type hypersensitivity reactions. Gulati N, Suárez-FariÃ±as M, Correa da Rosa J, Krueger JG. F1000Research. 2015 4:149. (IHC; Human)

Full Text Article PubMed: 26236467 PMC: PMC4505786

Indoleamine 2,3-dioxygenase regulates anti-tumor immunity in lung cancer by metabolic reprogramming of immune cells in the tumor microenvironment. Schafer CC, Wang Y, Hough KP, Sawant A, Grant SC, Thannickal VJ, Zmijewski J, Ponnazhagan S, Deshane JS. Oncotarget. 2016 November;7:75407-75424.

Full Text Article PubMed: 27705910 PMC: PMC5340181

IL-32 induces indoleamine 2,3-dioxygenase+CD1c+ dendritic cells and indoleamine 2,3-dioxygenase+CD163+ macrophages: Relevance to mycosis fungoides progression. Ohmatsu H, Humme D, Gonzalez J, Gulati N, Möbs M, Sterry W, Krueger JG. Oncoimmunology. 2016 May;6:e1181237.

Full Text Article PubMed: 28344860 PMC: PMC5353917

Submit a Publication for LS-C153780