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Catalog Number	Size	Price
LS-C745260-25	25 µl (0.95 mg/ml)	$304

IDO1 / IDO Antibody - Immunohistochemistry of Mouse Anti-IDO1 Antibody. Tissue: epididymis from wild-type (left) or IDO1 null mice (right). Fixation: frozen sections. Antigen retrieval: not required. Primary antibody: IDO1 (2E2) monoclonal antibody. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: IDO-1 is located in the cytosol. Staining: IDO-1 as precipitated brown signal.

IDO1 / IDO Antibody - Immunohistochemistry of Mouse anti-IDO1 antibody. Tissue: epididymis from wild-type (left) or IDO1 null mice (right). Fixation: paraffin-embedded. Primary antibody: IDO1 (2E2) monoclonal antibody. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: IDO-1 is located in the cytosol. Staining: IDO1 as precipitated brown signal.

IDO1 / IDO Antibody - Immunofluorescence Microscopy of Mouse anti-IDO1 Antibody. Cells: HEK293 cells. Fixation: 0.5% PFA. Expressing: mouse IDO-1 (left) and mouse IDO-2 (right). Primary antibody: IDO1 (2E2) monoclonal antibody. Antigen retrieval: not required. Secondary antibody: mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: IDO-1 is located in the cytosol. Staining: IDO1 as red fluorescent signal with bis-benzimide nuclear counterstain (blue).

IDO1 / IDO Antibody - Western Blot of mouse anti-IDO1 antibody. Lane 1: HEK293 control vector. Lane 2: HEK293 expressing mouse IDO1. Lane 3: HEK293 expressing mouse IDO2. Load: 35 µg per lane. Primary antibody: IDO 1 antibody at 1:400 for overnight at 4°C. Secondary antibody: mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 45.6 kDa, ~44 kDa for IDO1. Other band(s): unspecifics.

IDO1 / IDO Antibody - Western Blot of Mouse Anti-IDO1 antibody. Extracts from 293HEK Cells expressing: Lane 1: Control Vector. Lane 2: His-tagged mouse IDO1. Lane 3: mouse IDO1. Lane 4: His-tagged mouse IDO2. Lane 5: mouse IDO2. Lane 6: Epididymis from IDO null. Lane 7: wild type mice. Primary antibody: IDO-1(2E2) monoclonal antibody. Secondary antibody: mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 1xPBST overnight at 4°C. Predicted/Observed size: 41-42 kDa/41-42 kDa for IDO-1. Other band(s): none.

IDO1 / IDO Antibody - Flow Cytometry of Mouse anti-IDO1 antibody. Cells: HEK293 cells. Expresing: mouse IDO-1(blue) and mouse IDO-2 (red). Primary antibody: IDO1 (2E2) monoclonal antibody. Secondary antibody: Biotin mouse secondary antibody at 1:10,000 for 45 min at RT and streptavidin PE at 1:5,000 for 30 min at RT.

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Monoclonal Mouse anti‑Mouse IDO1 / IDO Antibody (clone 2E2.6, IHC, IF, WB) LS‑C745260

Name: Anti-IDO1 / IDO Antibody | Mouse anti-Mouse Monoclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C745260-25
Price: 304.0000 USD
Availability: InStock

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Antibody:

IDO1 / IDO Mouse anti-Mouse Monoclonal (2E2.6) Antibody

Application:

IHC, IF, WB, IP, Flo, ELISA

Reactivity:

Mouse

Format:

Unconjugated, Unmodified

$304

LS-C745260-25

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

IDO1 / IDO Mouse anti-Mouse Monoclonal (2E2.6) Antibody

Application:

IHC, IF, WB, IP, Flo, ELISA

Reactivity:

Mouse

Format:

Unconjugated, Unmodified

Specifications

Description

IDO antibody LS-C745260 is an unconjugated mouse monoclonal antibody to mouse IDO (IDO1). Validated for ELISA, Flow, IF, IHC, IP and WB.

Target

Mouse IDO1 / IDO

Synonyms

See All IDO1 / IDO Antibodies

Host

Mouse

Reactivity

Mouse (tested or 100% immunogen sequence identity)

Non-Reactivity

Human

Clonality

IgG1 Monoclonal

Clone

2E2.6

Conjugations

Unconjugated

Purification

Protein G affinity chromatography

Modifications

Unmodified

Immunogen

IDO1 antibody was produced in mouse by repeated immunizations with mouse recombinant IDO1 protein followed by hybridoma development.

Applications

IHC
Immunofluorescence (1:50 - 1:100)
Western blot (1:500 - 1:1500)
Immunoprecipitation
Flow Cytometry
ELISA (1:5000 - 1:50000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Applications should be user optimized.

Presentation

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Storage

Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About IDO1 / IDO

Uniprot: P14902 NCBI: NM_002164 AAA36081.1