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Catalog Number Size Price
LS-C331595-20 20 µl $287 
LS-C331595-100 100 µl $431 
IDE Antibody - Immunofluorescence analysis of A549 cells using IDE antibody. Blue: DAPI for nuclear staining.
IDE Antibody - Western blot analysis of extracts from normal (control) and IDE knockout (KO) HeLa cells, using IDE antibodyat 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 10s.
IDE Antibody - Western blot analysis of extracts of various cell lines, using IDE antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection.
IDE Antibody - Western blot analysis of extracts of various tissues.
IDE Antibody - Immunofluorescence analysis of A549 cells using IDE antibody. Blue: DAPI for nuclear staining.
IDE Antibody - Western blot analysis of extracts from normal (control) and IDE knockout (KO) HeLa cells, using IDE antibodyat 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 10s.
IDE Antibody - Western blot analysis of extracts of various cell lines, using IDE antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection.
IDE Antibody - Western blot analysis of extracts of various tissues.
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Polyclonal Rabbit anti‑Human IDE Antibody (IF, WB) LS‑C331595

Polyclonal Rabbit anti‑Human IDE Antibody (IF, WB) LS‑C331595

Antibody:
IDE Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$287
LS-C331595-20
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
IDE Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
IDE antibody LS-C331595 is an unconjugated rabbit polyclonal antibody to IDE from human. It is reactive with human and mouse. Validated for IF and WB.
Target
Human IDE
Synonyms
IDE | Abeta-degrading protease | Insulinase | INSULYSIN | Insulin protease | Insulin-degrading enzyme
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human IDE (NP_004960.2). MRYRLAWLLHPALPSTFRSVLGARLPPPERLCGFQKKTYSKMNNPAIKRIGNHITKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIAGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVRQELLKFHSAYYS
Specificity
Human IDE
Applications
  • Immunofluorescence (1:10 - 1:100)
  • Western blot (1:500 - 1:2000)
Usage
The predicted MW is 54kDa/117kDa, while the observed MW by Western blot was 118kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IDE
P14735 NM_004969 NP_004960.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024