Human ICAM-1 / CD54

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

84H10

Unconjugated

Protein A purified

Unmodified

K562 cell line.

Recognizes the D1 domain of ICAM-1. Reacts with the ICAM-1 antigen found in low levels on lymphocytes and strongly expressed on monocytes and granulocytes. This molecule is inducible to high levels by mitogenic lectins on lymphocytes and by IL-1 beta or IFN gamma on other cell types such as fibroblasts and endothelial cells. Detects an antigen of 90kD. Clone 84H10 has been reported to block ICAM1 mediated cellular adhesion and block binding of LFA-1 and P. falciparum to ICAM-1. Removal of sodium azide is recommended prior to use in functional assays - XXX recommend the use of EQU003 for this purpose.

• IHC
• IHC - Frozen (1:20 - 1:50)
• Immunoprecipitation
• Flow Cytometry (1:1 - 1:10)
• ELISA

• IHC - Paraffin

Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul.

PBS, 0.09% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P05362 NCBI: NM_000201 NP_000192.2