Rat ICAM-1 / CD54

Mouse

Rat (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

1A29

Unconjugated. Also available conjugated with FITC.

Protein G purified

Unmodified. Also available Low Endotoxin.

Rat Ax cells (a HEV derived cell line).

Recognizes the rat CD54 cell surface antigen, also known as intercellular adhesion molecule-1 (ICAM-1), a 90kD adhesion molecule belonging to the immunoglobulin superfamily. CD54 is a cell surface ligand of the lymphocyte integrin, LFA-1 and is known to play an important role in various cell-cell interactions in the immune system. Recent studies suggest that cross-linking of ICAM-1 using clone 1A29 induces calcium signalling. Functionally 1A29 inhibits homotypic aggregation of PHA blasts.

• IHC
• IHC - Paraffin
• IHC - Frozen
• Western blot
• Immunoprecipitation
• Flow Cytometry (1:25 - 1:50)

Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Immunohistology: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

PBS, 0.09% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P05362 NCBI: NM_000201 NP_000192.2