Target
Human ICAM-1 / CD54
Synonyms
ICAM1 | BB2 | CD54 antigen | CD54 | ICAM-1 | p3.58
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence SNCPDGQSTAKT, from the internal region of the protein sequence according to NP_000192.2.
Specificity
Human ICAM1 / ICAM-1 / CD54. The immunizing peptide represents part of the extracellular domain, and it does not overlap any known glycosite.
Applications
- Immunofluorescence (5 µg/ml)
- Western blot (1 - 3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:1000)
Usage
Peptide ELISA: antibody detection limit dilution 1:1000. Western blot: Approx 100kD band observed in lysates of cell lines Daudi and HeLa (calculated MW of 57.8kD according to NP_000192.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pino et al, Reproduction. 2009 Nov;138(5):837-47. PMID: 19661147). Recommended concentration: 1-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee