Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Location


Corporate Headquarters

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Telephone Numbers



Customer Service: (800) 227-6666 / (650) 697-3600


Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C186519-100 100 µg (0.5 mg/ml) $503 
ICAM-1 / CD54 Antibody - ICAM-1 / CD54 antibody immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
ICAM-1 / CD54 Antibody - ICAM-1 / CD54 antibody (2µg/ml) staining of NIH3T3 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ICAM-1 / CD54 Antibody - ICAM-1 / CD54 antibody (2µg/ml) staining of HeLa (A) and Daudi (B) cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ICAM-1 / CD54 Antibody - ICAM1 antibody (2 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ICAM-1 / CD54 Antibody - ICAM-1 / CD54 antibody immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
ICAM-1 / CD54 Antibody - ICAM-1 / CD54 antibody (2µg/ml) staining of NIH3T3 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ICAM-1 / CD54 Antibody - ICAM-1 / CD54 antibody (2µg/ml) staining of HeLa (A) and Daudi (B) cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ICAM-1 / CD54 Antibody - ICAM1 antibody (2 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
1 of 4
2 of 4
3 of 4
4 of 4

Polyclonal Goat anti‑Human ICAM‑1 / CD54 Antibody (aa94‑105, IF, WB) LS‑C186519

Polyclonal Goat anti‑Human ICAM‑1 / CD54 Antibody (aa94‑105, IF, WB) LS‑C186519

Antibody:
ICAM-1 / CD54 Goat anti-Human Polyclonal (aa94-105) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$503
LS-C186519-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ICAM-1 / CD54 Goat anti-Human Polyclonal (aa94-105) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CD54 antibody LS-C186519 is an unconjugated goat polyclonal antibody to human CD54 (ICAM-1) (aa94-105). Validated for IF, Peptide-ELISA and WB.
Target
Human ICAM-1 / CD54
Synonyms
ICAM1 | BB2 | CD54 antigen | CD54 | ICAM-1 | p3.58
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence SNCPDGQSTAKT, from the internal region of the protein sequence according to NP_000192.2.
Epitope
aa94-105
Specificity
Human ICAM1 / ICAM-1 / CD54. The immunizing peptide represents part of the extracellular domain, and it does not overlap any known glycosite.
Applications
  • Immunofluorescence (5 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:1000)
Usage
Peptide ELISA: antibody detection limit dilution 1:1000. Western blot: Approx 100kD band observed in lysates of cell lines Daudi and HeLa (calculated MW of 57.8kD according to NP_000192.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pino et al, Reproduction. 2009 Nov;138(5):837-47. PMID: 19661147). Recommended concentration: 1-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ICAM-1 / CD54
P05362 NM_000201 NP_000192.2

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/2/2024