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Catalog Number Size Price
LS-C313412-10 10 µg $318 
LS-C313412-100 100 µg (0.5 mg/ml) $470 
ICAM-1 / CD54 Antibody - ICAM1 / CD54 antibody. IHC(P): Rat Intestine Tissue.
ICAM-1 / CD54 Antibody - ICAM1 / CD54 antibody. IHC(P): Rat Spleen Tissue.
ICAM-1 / CD54 Antibody - ICAM1 / CD54 antibody. IHC(P): Mouse Spleen Tissue.
ICAM-1 / CD54 Antibody - ICAM1 / CD54 antibody. IHC(P): Mouse Intestine Tissue.
ICAM-1 / CD54 Antibody - ICAM1 / CD54 antibody. IHC(P): Mouse Lung Tissue.
ICAM-1 / CD54 Antibody - Western blot analysis of ICAM1 using anti-ICAM1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse testicular tissue lysates, Lane 2: mouse thymus tissue lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICAM1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ICAM1 at approximately 90KD. The expected band size for ICAM1 is at 58KD.
ICAM-1 / CD54 Antibody - WB of ICAM1 / CD54 antibody. All lanes: Anti-ICAM1 at 0.5ug/ml. Lane 1: Rat Thymus Tissue Lysate at 40ug. Lane 2: Rat Spleen Tissue Lysate at 40ug. Lane 3: PC12 Whole Cell Lysate at 40ug. Lane 4: NRK Whole Cell Lysate at 40ug. Lane 5: RH35 Whole Cell Lysate at 40ug. Predicted bind size: 58KD. Observed bind size: 90KD.
ICAM-1 / CD54 Antibody - ICAM1 / CD54 antibody. IHC(P): Rat Intestine Tissue.
ICAM-1 / CD54 Antibody - ICAM1 / CD54 antibody. IHC(P): Rat Spleen Tissue.
ICAM-1 / CD54 Antibody - ICAM1 / CD54 antibody. IHC(P): Mouse Spleen Tissue.
ICAM-1 / CD54 Antibody - ICAM1 / CD54 antibody. IHC(P): Mouse Intestine Tissue.
ICAM-1 / CD54 Antibody - ICAM1 / CD54 antibody. IHC(P): Mouse Lung Tissue.
ICAM-1 / CD54 Antibody - Western blot analysis of ICAM1 using anti-ICAM1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse testicular tissue lysates, Lane 2: mouse thymus tissue lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICAM1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ICAM1 at approximately 90KD. The expected band size for ICAM1 is at 58KD.
ICAM-1 / CD54 Antibody - WB of ICAM1 / CD54 antibody. All lanes: Anti-ICAM1 at 0.5ug/ml. Lane 1: Rat Thymus Tissue Lysate at 40ug. Lane 2: Rat Spleen Tissue Lysate at 40ug. Lane 3: PC12 Whole Cell Lysate at 40ug. Lane 4: NRK Whole Cell Lysate at 40ug. Lane 5: RH35 Whole Cell Lysate at 40ug. Predicted bind size: 58KD. Observed bind size: 90KD.
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Polyclonal Rabbit anti‑Mouse ICAM‑1 / CD54 Antibody (aa198‑537, IHC, WB) LS‑C313412

Polyclonal Rabbit anti‑Mouse ICAM‑1 / CD54 Antibody (aa198‑537, IHC, WB) LS‑C313412

Note: This antibody replaces LS-C388651
Antibody:
ICAM-1 / CD54 Rabbit anti-Mouse Polyclonal (aa198-537) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C313412-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ICAM-1 / CD54 Rabbit anti-Mouse Polyclonal (aa198-537) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CD54 antibody LS-C313412 is an unconjugated rabbit polyclonal antibody to CD54 (ICAM-1) (aa198-537) from mouse. It is reactive with mouse and rat. Validated for IHC and WB.
Target
Mouse ICAM-1 / CD54
Synonyms
ICAM1 | BB2 | CD54 antigen | CD54 | ICAM-1 | p3.58
Host
Rabbit
Reactivity
Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived mouse ICAM1 recombinant protein (Position: G198-P537). Mouse ICAM1 shares 74% amino acid (aa) sequence identity with rat ICAM1.
Epitope
aa198-537
Specificity
Expressed at low level on a subpopulation of lymphocytes, macrophages, and endothelial cells, but is strongly induced on these cells, and on fibroblasts and epithelial cells.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ICAM-1 / CD54
P05362 NM_000201 NP_000192.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/2/2024