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Antibodies > HSP90AB1 / HSP90 Alpha B1 > LS-C800985
 
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Catalog Number Size Price
LS-C800985-100 100 µl (1 mg/ml) $379 
LS-C800985-200 200 µl (1 mg/ml) $421 
HSP90AB1 / HSP90 Alpha B1 Antibody - 1:200 staining rat liver tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HSP90AB1 / HSP90 Alpha B1 Antibody - 1:200 staining rat kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HSP90AB1 / HSP90 Alpha B1 Antibody - 1:200 staining rat kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HSP90AB1 / HSP90 Alpha B1 Antibody - 1:200 staining rat lung tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HSP90AB1 / HSP90 Alpha B1 Antibody - 1:200 staining rat lung tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HSP90AB1 / HSP90 Alpha B1 Antibody - 1:200 staining rat liver tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HSP90AB1 / HSP90 Alpha B1 Antibody - 1:200 staining mouse spleen tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HSP90AB1 / HSP90 Alpha B1 Antibody - 1:200 staining mouse kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HSP90AB1 / HSP90 Alpha B1 Antibody - 1:200 staining mouse kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HSP90AB1 / HSP90 Alpha B1 Antibody - 1:200 staining mouse testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HSP90AB1 / HSP90 Alpha B1 Antibody - 1:200 staining mouse testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HSP90AB1 / HSP90 Alpha B1 Antibody - 1:200 staining mouse spleen tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HSP90AB1 / HSP90 Alpha B1 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
HSP90AB1 / HSP90 Alpha B1 Antibody - Staining HeLa cells treated with TNF-a 20nM 15' cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
HSP90AB1 / HSP90 Alpha B1 Antibody - Western blot analysis of HSP90B phosphorylation expression in TNF-a treated HeLa whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
HSP90AB1 / HSP90 Alpha B1 Antibody - Western blot analysis of HSP90B phosphorylation expression in mouse brain tissue lysates. The lane on the right is treated with the antigen-specific peptide.
HSP90AB1 / HSP90 Alpha B1 Antibody - 1:200 staining rat liver tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HSP90AB1 / HSP90 Alpha B1 Antibody - 1:200 staining rat kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HSP90AB1 / HSP90 Alpha B1 Antibody - 1:200 staining rat kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HSP90AB1 / HSP90 Alpha B1 Antibody - 1:200 staining rat lung tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HSP90AB1 / HSP90 Alpha B1 Antibody - 1:200 staining rat lung tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HSP90AB1 / HSP90 Alpha B1 Antibody - 1:200 staining rat liver tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HSP90AB1 / HSP90 Alpha B1 Antibody - 1:200 staining mouse spleen tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HSP90AB1 / HSP90 Alpha B1 Antibody - 1:200 staining mouse kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HSP90AB1 / HSP90 Alpha B1 Antibody - 1:200 staining mouse kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HSP90AB1 / HSP90 Alpha B1 Antibody - 1:200 staining mouse testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HSP90AB1 / HSP90 Alpha B1 Antibody - 1:200 staining mouse testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HSP90AB1 / HSP90 Alpha B1 Antibody - 1:200 staining mouse spleen tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
HSP90AB1 / HSP90 Alpha B1 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
HSP90AB1 / HSP90 Alpha B1 Antibody - Staining HeLa cells treated with TNF-a 20nM 15' cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
HSP90AB1 / HSP90 Alpha B1 Antibody - Western blot analysis of HSP90B phosphorylation expression in TNF-a treated HeLa whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
HSP90AB1 / HSP90 Alpha B1 Antibody - Western blot analysis of HSP90B phosphorylation expression in mouse brain tissue lysates. The lane on the right is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human HSP90AB1 / HSP90 Alpha B1 Antibody (phospho‑Ser254, IHC, IF, WB) LS‑C800985

Polyclonal Rabbit anti‑Human HSP90AB1 / HSP90 Alpha B1 Antibody (phospho‑Ser254, IHC, IF, WB) LS‑C800985

Submit Review LSBio Guarantee
Antibody:
HSP90AB1 / HSP90 Alpha B1 Rabbit anti-Human Polyclonal (pSer254) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C800985-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
HSP90AB1 / HSP90 Alpha B1 Rabbit anti-Human Polyclonal (pSer254) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
HSP90 Alpha B1 antibody LS-C800985 is an unconjugated rabbit polyclonal antibody to HSP90 Alpha B1 (HSP90AB1) (pSer254) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human HSP90AB1 / HSP90 Alpha B1
Synonyms
HSP90AB1 | D6S182 | Heat shock 84 kDa | Heat shock protein beta | Heat shock protein HSP 90-beta | HSP 84 | HSP84 | HSP 90 | HSP90-BETA | HSP90B | HSPC2 | HSPCB
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human HSP90B around the phosphorylation site of Serine 254.
Epitope
pSer254
Specificity
Phospho-HSP90B (Ser254) Antibody detects endogenous levels of HSP90B only when phosphorylated at Serine 254.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSP90AB1 / HSP90 Alpha B1
P08238 NM_007355 NP_031381.2

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HSP90AB1 / HSP90 Alpha B1 Antibody (aa449‑481) LS‑C407848
Species: Human, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Sheep
Applications: IHC, IHC - Paraffin, Western blot
HSP90AB1 / HSP90 Alpha B1 Antibody - Immunohistochemical analysis of HSP90 beta (pS226) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
HSP90AB1 / HSP90 Alpha B1 Antibody (phospho‑Ser226) LS‑C359019
Species: Human, Monkey, Mouse, Rat, Bovine
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
See all HSP90AB1 / HSP90 Alpha B1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 1/11/2025
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