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Antibodies > HMG2 / HMGB2 > LS-C368661
 
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Catalog Number Size Price
LS-C368661-30 30 µl (1 mg/ml) $255 
LS-C368661-50 50 µl $271 
LS-C368661-100 100 µl (1 mg/ml) $299 
LS-C368661-200 200 µl (1 mg/ml) $351 
HMG2 / HMGB2 Antibody - Immunohistochemical analysis of HMGB2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
HMG2 / HMGB2 Antibody - Immunofluorescent analysis of HMGB2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
HMG2 / HMGB2 Antibody - Western blot analysis of HMGB2 expression in HeLa (A); K562 (B) whole cell lysates.
HMG2 / HMGB2 Antibody - Immunohistochemical analysis of HMGB2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
HMG2 / HMGB2 Antibody - Immunofluorescent analysis of HMGB2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
HMG2 / HMGB2 Antibody - Western blot analysis of HMGB2 expression in HeLa (A); K562 (B) whole cell lysates.
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Polyclonal Rabbit anti‑Human HMG2 / HMGB2 Antibody (Internal, IHC, IF, WB) LS‑C368661

Polyclonal Rabbit anti‑Human HMG2 / HMGB2 Antibody (Internal, IHC, IF, WB) LS‑C368661

Submit Review LSBio Guarantee
Antibody:
HMG2 / HMGB2 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Bovine
Format:
Unconjugated, Unmodified
Price
Catalog Number
$255
LS-C368661-30
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
HMG2 / HMGB2 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Bovine
Format:
Unconjugated, Unmodified

Specifications

Description
HMGB2 antibody LS-C368661 is an unconjugated rabbit polyclonal antibody to HMGB2 (HMG2) (Internal) from human. It is reactive with human, mouse and bovine. Validated for ICC, IF, IHC and WB.
Target
Human HMG2 / HMGB2
Synonyms
HMGB2 | HMG-2 | High mobility group box 2 | High-mobility group box 2 | HMG2 | High mobility group protein 2 | High mobility group protein B2
Host
Rabbit
Reactivity
Human, Mouse, Bovine (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human HMGB2.
Epitope
Internal
Specificity
Recognizes endogenous levels of HMGB2 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HMG2 / HMGB2
P26583 NM_002129 NP_002120.1

Publications (0)

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Featured Products

HMG2 / HMGB2 Antibody - Anti-HMGB2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ HMG2 / HMGB2 Antibody (clone 3D2) LS‑B4355
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
HMG2 / HMGB2 Antibody - HMGB2 antibody ARP31939_P050-NP_002120-HMGB2 (high-mobility group box 2) Antibody IHC of formalin-fixed, paraffin-embedded human Liver. Positive label: Hepatocytes indicated with arrows. Antibody concentration 4-8 ug/ml. Magnification 400X. This image was taken for the unconjugated form of this product. Other forms have not been tested.
HMG2 / HMGB2 Antibody (C‑Terminus) LS‑C29675
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
HMG2 / HMGB2 Antibody - Anti-HMGB2 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IHC‑plus™ HMG2 / HMGB2 Antibody (clone 3C7) LS‑B4354
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
HMG2 / HMGB2 Antibody - HMGB2 Antibody western blot of HL-60,K562,H-4-II-E cell line lysates (35 ug/lane). The HMGB2 antibody detected the HMGB2 protein (arrow).
HMG2 / HMGB2 Antibody (aa92‑118) LS‑C99091
Species: Human
Applications: Western blot
HMG2 / HMGB2 Antibody - Immunofluorescence of monoclonal antibody to HMGB2 on HeLa cell (antibody concentration 10 ug/ml).
HMG2 / HMGB2 Antibody (clone 3E5) LS‑C104971
Species: Human
Applications: Immunofluorescence, Western blot, ELISA
HMG2 / HMGB2 ELISA Kit
Human HMG2 / HMGB2 (Sandwich ELISA) ELISA Kit - LS-F11643
Reactivity: Human
Range: 0.312-20 ng/ml
See all HMG2 / HMGB2 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 2/5/2025
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