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Catalog Number Size Price
LS-C662937-10 10 µg $318 
LS-C662937-100 100 µg $470 
HMBS / PBGD Antibody - Western blot analysis of HMBS using anti-HMBS antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,Lane 2: human placenta tissue lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human 22RV1 whole cell lysates,Lane 5: human U20S whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMBS antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HMBS at approximately 45KD. The expected band size for HMBS is at 39KD.
HMBS / PBGD Antibody - Western blot analysis of HMBS using anti-HMBS antibody
HMBS / PBGD Antibody - Western blot analysis of HMBS using anti-HMBS antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,Lane 2: human placenta tissue lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human 22RV1 whole cell lysates,Lane 5: human U20S whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMBS antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HMBS at approximately 45KD. The expected band size for HMBS is at 39KD.
HMBS / PBGD Antibody - Western blot analysis of HMBS using anti-HMBS antibody
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Polyclonal Rabbit anti‑Human HMBS / PBGD Antibody (WB) LS‑C662937

Polyclonal Rabbit anti‑Human HMBS / PBGD Antibody (WB) LS‑C662937

Antibody:
HMBS / PBGD Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662937-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
HMBS / PBGD Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
PBGD antibody LS-C662937 is an unconjugated rabbit polyclonal antibody to human PBGD (HMBS). Validated for ELISA and WB.
Target
Human HMBS / PBGD
Synonyms
HMBS | Hydroxymethylbilane synthase | PBG-D | PBGD | PORC | Porphyria, acute Chester type | Porphobilinogen deaminase | Pre-uroporphyrinogen synthase | UPS | Uroporphyrinogen I synthetase | Uroporphyrinogen I synthase
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Immunogen
E. coli-derived human HMBS recombinant protein (Position: N6-H361).
Specificity
Isoform 1 is ubiquitously expressed. Isoform 2 is found only in erythroid cells.
Applications
  • Western blot
  • ELISA
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HMBS / PBGD
P08397 NM_000190 NP_000181.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/22/2024