Reactivity
Human, Monkey, Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Immunogen
Synthetic peptide corresponding to the C-Terminus of human histone H2B protein in which Lys120 is mono-ubiquitylated.
Specificity
Endogenous levels of histone H2B, ubiquitylated on Lys120. Species Crossreactivity: monkey, mouse, rat.
Usage
Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Presentation
10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 100ug/ml BSA, 0.02% Sodium Azide, 50% Glycerol
Storage
May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for up to 1 year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee