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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2446-50 | 50 µl | $525 |
IHC‑plus™ Polyclonal Rabbit anti‑Human Histone H2B Antibody (Acetyl‑Lys12, IHC, WB) LS‑B2446
IHC‑plus™ Polyclonal Rabbit anti‑Human Histone H2B Antibody (Acetyl‑Lys12, IHC, WB) LS‑B2446
Note: This antibody replaces LS-C24283
Antibody:
Histone H2B Rabbit anti-Human Polyclonal (Acetyl-Lys12) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat, Chicken
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
Histone H2B Rabbit anti-Human Polyclonal (Acetyl-Lys12) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat, Chicken
Format:
Unconjugated, Unmodified
Specifications
Description
Histone H2B antibody LS-B2446 is an unconjugated rabbit polyclonal antibody to Histone H2B (Acetyl-Lys12) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Host
Rabbit
Reactivity
Human, Mouse, Rat, Chicken
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Immunogen
KLH-conjugated, synthetic peptide APAPKAcKGSKKAITK corresponding to 7-20 amino acid of human Histone H2B member F. The immunizing sequence has 13 out of 14 identical amino acids in mouse, chicken and rat.
Epitope
Acetyl-Lys12
Specificity
Recognizes human acetylated Histone 2B, Mr 15kD. Species sequence Homology: mouse, chicken and rat.
Applications
- IHC
- IHC - Paraffin (1:100)
- Western blot (1:1000 - 1:10000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2446 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2446 was determined to be 1:100.
Presentation
Serum, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Validation
Anti-Histone H2B antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-Histone H2B antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
See More About...
LSBio Ratings
IHC-plus™ Histone H2B Antibody (Acetyl-Lys12) for IHC, WB/Western LS-B2446 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Customer Reviews (0)
Featured Products
Species:
Human, Mouse, Rat, Bovine, Chicken, Zebrafish, Drosophila, Insect
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Bovine
Applications:
Immunofluorescence, Western blot, Immunoprecipitation
Species:
Human, Mouse, Rat, Bovine
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/25/2024
Date Requested: 11/25/2024
