Human Histone H2B

Rabbit

Human, Mouse, Rat, Bovine, Chicken, Zebrafish, Drosophila, Insect (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Protein G purified

Unmodified

This antibody was generated by immunizing rabbits with a synthetic peptide corresponding to amino acid residues 111-125 AVSEGTKAVTKYTSSof human Histone H2B; GenBank no. CAI19747.1. This peptide sequence is 100% conserved between multiple species including human, mouse, rat, dog, chimpanzee, cow, chicken, duck, zebrafish, and drosophila. Percent identity by BLAST analysis: Human, Orangutan, Mouse, Rat, Bovine, Chicken, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito, Nematode (100%).

aa111-125

A 15 kD band is observed. This is routinely quality controlled in human lysates.

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (2 µg/ml)

Immunohistochemistry: LS-B2035 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2035 was determined to be 5 ug/ml.

PBS, 0.05% Sodium Azide, 0.05% BSA

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.