Target
Hemagglutinin / HA Tag
Clonality
IgG3
Monoclonal
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Protein A purified
Immunogen
Synthetic peptide containing the sequence aa98-106 (YPYDVPDYA) derived from the influenza virus hemaglutinin (HA) protein (KLH).
Specificity
Recognizes HA tagged (YPYDVPDYA) proteins overexpressed in cells, including both amino or carboxy-termini of targeted proteins in transfected mammalian cells.
Applications
- ICC (5 µg/ml)
- Western blot (0.5 - 2 µg/ml)
- Immunoprecipitation (5 µg/ml)
Usage
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 0.5-2 ug/ml. Detects recombinant protein containing the HA-tag at the N-terminus in RIPA lysates from HEK293 cells. Immunoprecipitation: 5 ug/ml. Reported to immunoprecipitate recombinant HA-tagged fusion protein. Immunocytochemistry: 5 ug/ml. Reported to immunostain HA-tagged fusion protein.
Presentation
PBS, pH 7.4, 0.05% Sodium Azide, 40% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee