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Hemagglutinin / HA Tag

HA Tag / Hemagglutinin Tag

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. The amino acid sequence is: YPYDVPDYA

Hemagglutinin / HA Tag Target Details

Target Name: HA Tag / Hemagglutinin Tag
Target Abbreviation: HA Tag

Publications (4)

1
Immunization with reverse-genetics-produced H5N1 influenza vaccine protects ferrets against homologous and heterologous challenge. Govorkova EA, Webby RJ, Humberd J, Seiler JP, Webster RG. The Journal of infectious diseases. 2006 194:159-67. [PubMed:16779721]
2
Passive immunoprophylaxis and therapy with humanized monoclonal antibody specific for influenza A H5 hemagglutinin in mice. Hanson BJ, Boon AC, Lim AP, Webb A, Ooi EE, Webby RJ. Respiratory research. 2006 7:126. [PubMed:17040574] [PMC:PMC1626464]
3
Amino acid residues in the fusion peptide pocket regulate the pH of activation of the H5N1 influenza virus hemagglutinin protein. Reed ML, Yen HL, DuBois RM, Bridges OA, Salomon R, Webster RG, Russell CJ. Journal of virology. 2009 83:3568-80. [PubMed:19193808] [PMC:PMC2663236]
4
FOXO3 modulates endothelial gene expression and function by classical and alternative mechanisms. Czymai T, Viemann D, Sticht C, Molema G, Goebeler M, Schmidt M. The Journal of biological chemistry. 2010 285:10163-78. [PubMed:20123982] [PMC:PMC2856222] Related Antibodies: LS-C157795.
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☰ Filters
Antibodies (120)
Primary (120)
Hemagglutinin / HA Tag (120)
Human (4)
Mouse (6)
All species (2)
Influenza Virus (2)
Rabbit (2)
Virus (2)
IHC (20)
IHC-Fr (1)
IHC-P (2)
WB (109)
Flo (8)
ChrIP (2)
ELISA (79)
ICC (19)
IF (20)
IP (35)
Micro (2)
rabbit (51)
mouse (54)
goat (7)
chicken (7)
sheep (1)
Ig (1)
IgG (42)
IgG1 (10)
IgG1,k (5)
IgG2a (7)
IgG2a,k (1)
IgG2b (9)
IgG3 (7)
IgG3,k (1)
IgY (5)
monoclonal mc (56)
polyclonal pc (63)
recombinant monoclonal rmc (1)
10 (1)
109B2 (2)
12CA5 (5)
16B12 (5)
1809CT823.37.18 (1)
4G3 (1)
5D8 (1)
5E11D8 (3)
AT5E7 (1)
HA.C5 (1)
Agarose Conjugated (1)
APC Conjugated (5)
Biotin (NHS-LC) Conjugated (1)
Biotin Conjugated (11)
FITC Conjugated (9)
HiLyte Fluor 488 Conjugated (1)
HiLyte Fluor 647 Conjugated (1)
HRP Conjugated (18)
PE Conjugated (6)
Preservative Free (2)
RPE Conjugated (1)
Unconjugated (66)
C-Terminus (9)
aa114-122 (3)
Internal (2)
aa98-106 (1)
No (119)
Yes (1)
Hemagglutinin / HA Tag Antibody - Anti-HA Epitope Tag Antibody - Immunohistochemistry. Affinity Purified anti-HA epitope tag polyclonal antibody detects HA tagged recombinant proteins by IHC on formalin fixed paraffin embedded tissue. Arrowheads point to expression of HA tagged proteins in endothelial cells of mouse aorta. Sections of 4 micron were prepared from representative paraffin blocks. Sections were then deparaffinized and rehydrated with xylene and alcohol. Citrate buffer antigen retrieval was performed for 30 min in a boiling jar. Anti-HA was diluted in blocking buffer at 1:2000 and reacted at 4° C overnight followed by signal detection using horseradish peroxidase with DAB as the chromogenic substrate. Tissue was counterstained with Mayers hematoxylin. Personal Communication, Behzad Yeganeh, U. Manitoba, Winnipeg, Canada.
Select
Hemagglutinin / HA Tag Rabbit Polyclonal (aa114-122) Antibody
ChrIP, ELISA, IHC, IP, WB
Unconjugated
100 µg/$435
Select
Hemagglutinin / HA Tag Rabbit anti-Virus Polyclonal (C-Terminus) Antibody
Virus
ELISA, IHC-P, WB
Unconjugated
50 µg/$384
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Hemagglutinin / HA Tag Sheep Polyclonal Antibody
IHC, WB
Unconjugated
500 µg/$393
Hemagglutinin / HA Tag Antibody - Detection of HA-tagged fusion protein in 200, 100, and 50ng of E. coli cell lysate
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Hemagglutinin / HA Tag Rabbit Polyclonal Antibody
ELISA, ICC, IP, WB
Unconjugated
100 µg/$509
Hemagglutinin / HA Tag Antibody - Immunocytochemistry/Immunofluorescence analysis of non-transfected CHO cells (a) or HA-tagged protein transfected CHO cells (b) using THE TM HA Tag Antibody [iFluor 488], mAb, Mouse.
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Hemagglutinin / HA Tag Mouse Monoclonal (HiLyte Fluor 488) Antibody
Flo, IF
HiLyte Fluor 488 Conjugated
100 µg/$512
Select
Hemagglutinin / HA Tag Mouse Monoclonal (C-Terminus) Antibody
Flo, ICC, IF, IHC, WB
Unconjugated
100 µl/$702
Hemagglutinin / HA Tag Antibody - Immunofluorescent analysis of HA-tag staining in 293T cells transfected with a HA-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Hemagglutinin / HA Tag Mouse Monoclonal Antibody
ICC, IF, IP, WB
Unconjugated
100 µl/$267
Select
Hemagglutinin / HA Tag Chicken Polyclonal (C-Terminus) Antibody
ELISA, IHC, WB
Unconjugated
1 ml/$717
Hemagglutinin / HA Tag Antibody - 293 cells transfected with C-terminal HA tag protein. Primary antibody: 1 ug/ml Anti-HA-tag Monoclonal Antibody (Mouse). Secondary antibody: 2 ug/ml Fluorescein Conjugated Affinity Purified Anti-mouse IgG (Rockland, 610-102-121).
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Hemagglutinin / HA Tag Mouse Monoclonal (5E11D8) Antibody
ELISA, Flo, IF, IP, WB
Unconjugated
100 µg/$461
Hemagglutinin / HA Tag Antibody - HA-tagged recombinant protein was expressed in COS7 cells. 15 ug of cell lysate was loaded onto a gel to test the HA tag antibody.
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Hemagglutinin / HA Tag Mouse Monoclonal Antibody
WB
Unconjugated
200 µl/$393
Hemagglutinin / HA Tag Antibody - Western blot of Multiple Tag using HA Tag Antibody [HRP], mAb, Mouse (0. 5 ug/ml). . Predicted Size: 52 kD. Observed Size: 52 kD. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Hemagglutinin / HA Tag Mouse Monoclonal (HRP) (5E11D8) Antibody
ELISA, WB
HRP Conjugated
100 µg/$474
Select
Hemagglutinin / HA Tag Goat Polyclonal (C-Terminus) (HRP) Antibody
ELISA, WB
HRP Conjugated
40 µg/$458; 100 µg/$557
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Hemagglutinin / HA Tag Goat Polyclonal Antibody
All species
WB
Unconjugated
800 µg/$422
Select
Hemagglutinin / HA Tag Goat Polyclonal (aa114-122) (FITC) Antibody
ELISA
FITC Conjugated
100 µl/$801
Hemagglutinin / HA Tag Antibody - GTPase activity of dynamin-2 is required for endocytosis of cell-surface tTG. MRC-5 fibroblasts were transiently transfected with either wild-type (wt) or a GTPase-deficient dynamin-2 mutant (K44A) with a hemagglutinin (HA) tag.(Provided by Evgeny A. Zemskov, Irina Mikhailenko:Journal of Cell Science 120, 3188-3199 (2007))
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Hemagglutinin / HA Tag Rabbit Polyclonal Antibody
ChrIP, IF, WB
Unconjugated
200 µl/$393
Select
Hemagglutinin / HA Tag Mouse Monoclonal Antibody
IHC, IP, WB
Unconjugated
100 µg/$545
Hemagglutinin / HA Tag Antibody - Immunofluorescence staining of HA tag Antibody, mAb, Rabbit with HA-tagged red fluorescent protein expression in CHO cells. The cells were fixed with 4% Poly-Formaldehyde for 5min, permeabilized with 0.1% TritonX-100 for 5 minutes, and blocked in 3% BSA for 30min at room temperature. The cells were then stained with 1/1000 Rabbit Anti-HA-tag mAb at room temperature for 2h, followed by a further incubation at 37 °C for 1h with Mouse Anti-Rabbit IgG Antibody [iFluor488], mAb at 5 µg/ml.
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Hemagglutinin / HA Tag Rabbit Monoclonal (109B2) Antibody
ELISA, IF, WB
Unconjugated
40 µg/$385
Select
Hemagglutinin / HA Tag Rabbit Polyclonal (C-Terminus) (Biotin) Antibody
ELISA, IHC, WB
Biotin Conjugated
100 µg/$845
Select
Hemagglutinin / HA Tag Rabbit Polyclonal (HRP) Antibody
ELISA, IHC, WB
HRP Conjugated
100 µg/$544
Select
Hemagglutinin / HA Tag Mouse Monoclonal (Biotin) Antibody
ICC, IP, WB
Biotin Conjugated
100 µl/$1,119
Select
Hemagglutinin / HA Tag Mouse Monoclonal (HRP) Antibody
ELISA, WB
HRP Conjugated
200 µl/$952
Select
Hemagglutinin / HA Tag Mouse Monoclonal (Biotin) Antibody
ELISA, WB
Biotin Conjugated
200 µl/$952
Select
Hemagglutinin / HA Tag Mouse Monoclonal (PE) (12CA5) Antibody
ELISA, WB
PE Conjugated
200 µl/$952
Select
Hemagglutinin / HA Tag Rabbit Polyclonal Antibody
IP, WB
Unconjugated
50 µg/$471
Select
Hemagglutinin / HA Tag Mouse anti-Influenza Virus Monoclonal Antibody
Influenza Virus
ELISA, Flo, ICC, IF, IP, WB
Unconjugated
20 µl/$271; 100 µl/$398; 500 µl/$586
Viewing 1-25 of 120 product results



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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).