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Catalog Number Size Price
LS-C312791-10 10 µg $318 
LS-C312791-100 100 µg $470 
HAVCR1 / KIM-1 Antibody - HAVCR1 / KIM-1 antibody. IHC(P): Rat Testis Tissue.
HAVCR1 / KIM-1 Antibody - Western blot analysis of KIM1 using anti-KIM1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: rat testis tissue lysates, Lane 3: rat heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIM1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KIM1 at approximately 50KD. The expected band size for KIM1 is at 39KD.
HAVCR1 / KIM-1 Antibody - WB of HAVCR1 / KIM-1 antibody. Lane 1: SMMC Cell Lysate. Lane 2: HELA Cell Lysate. Lane 3: PANC Cell Lysate. Lane 4: M231 Cell Lysate. Lane 5: M453 Cell Lysate.
HAVCR1 / KIM-1 Antibody - HAVCR1 / KIM-1 antibody. IHC(P): Rat Testis Tissue.
HAVCR1 / KIM-1 Antibody - Western blot analysis of KIM1 using anti-KIM1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: rat testis tissue lysates, Lane 3: rat heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIM1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KIM1 at approximately 50KD. The expected band size for KIM1 is at 39KD.
HAVCR1 / KIM-1 Antibody - WB of HAVCR1 / KIM-1 antibody. Lane 1: SMMC Cell Lysate. Lane 2: HELA Cell Lysate. Lane 3: PANC Cell Lysate. Lane 4: M231 Cell Lysate. Lane 5: M453 Cell Lysate.
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Polyclonal Rabbit anti‑Rat HAVCR1 / KIM‑1 Antibody (aa289‑307, IHC, WB) LS‑C312791

Polyclonal Rabbit anti‑Rat HAVCR1 / KIM‑1 Antibody (aa289‑307, IHC, WB) LS‑C312791

Note: This antibody replaces LS-C389339
Antibody:
HAVCR1 / KIM-1 Rabbit anti-Rat Polyclonal (aa289-307) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C312791-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
HAVCR1 / KIM-1 Rabbit anti-Rat Polyclonal (aa289-307) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB
Reactivity:
Rat
Format:
Unconjugated, Unmodified

Specifications

Description
KIM-1 antibody LS-C312791 is an unconjugated rabbit polyclonal antibody to rat KIM-1 (HAVCR1) (aa289-307). Validated for IHC and WB.
Target
Rat HAVCR1 / KIM-1
Synonyms
HAVCR1 | HAVCR-1 | KIM-1 | Kidney injury molecule 1 | KIM1 | T-cell membrane protein 1 | TIM1 | TIMD1 | TIM-1 | HAVCR | TIMD-1 | CD365
Host
Rabbit
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of rat TIM 1(289-307 aa HPRAEDNIYIIEDRSRGAE).
Epitope
aa289-307
Specificity
Expressed at a low level in normal kidney but are increased dramatically in postischemic kidney. Expressed in proliferating bromodeoxyuridine-positive and dedifferentiated vimentin-positive epithelial cells in regenerating proximal tubules. .
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HAVCR1 / KIM-1
Q96D42 NM_012206 NP_036338.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024