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Antibodies > GSTM1 > LS-C756622
 
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Catalog Number Size Price
LS-C756622-10 10 µg $318 
LS-C756622-100 100 µg $470 
GSTM1 Antibody - IHC analysis of GSTM1 using anti-GSTM1 Antibody. GSTM1 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml mouse anti-GSTM1 Antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
GSTM1 Antibody - IHC analysis of GSTM1 using anti-GSTM1 Antibody. GSTM1 was detected in paraffin-embedded section of rat liver tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml mouse anti-GSTM1 Antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
GSTM1 Antibody - Western blot analysis of GSTM1 using anti-GSTM1 Antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela, whole cell lysate, Lane 2: human T-47D whole cell lysate, Lane 3: rat brain tissue lysate, Lane 4: rat lung tissue lysate, Lane 5: rat stomach tissue lysate, Lane 6: mouse lung tissue lysate, Lane 7: mouse stomach tissue lysate, Lane 8: mouse kidney tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-GSTM1 antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GSTM1 at approximately 26KD. The expected band size for GSTM1 is at 26KD.
GSTM1 Antibody - Flow Cytometry analysis of HELA cells using anti-GSTM1 Antibody. Overlay histogram showing HELA cells stained with anti-GSTM1 Antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-GSTM1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
GSTM1 Antibody - Flow Cytometry analysis of U20S cells using anti-GSTM1 Antibody. Overlay histogram showing U20S cells stained with anti-GSTM1 Antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-GSTM1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
GSTM1 Antibody - IHC analysis of GSTM1 using anti-GSTM1 Antibody. GSTM1 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml mouse anti-GSTM1 Antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
GSTM1 Antibody - IHC analysis of GSTM1 using anti-GSTM1 Antibody. GSTM1 was detected in paraffin-embedded section of rat liver tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml mouse anti-GSTM1 Antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
GSTM1 Antibody - Western blot analysis of GSTM1 using anti-GSTM1 Antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela, whole cell lysate, Lane 2: human T-47D whole cell lysate, Lane 3: rat brain tissue lysate, Lane 4: rat lung tissue lysate, Lane 5: rat stomach tissue lysate, Lane 6: mouse lung tissue lysate, Lane 7: mouse stomach tissue lysate, Lane 8: mouse kidney tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-GSTM1 antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GSTM1 at approximately 26KD. The expected band size for GSTM1 is at 26KD.
GSTM1 Antibody - Flow Cytometry analysis of HELA cells using anti-GSTM1 Antibody. Overlay histogram showing HELA cells stained with anti-GSTM1 Antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-GSTM1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
GSTM1 Antibody - Flow Cytometry analysis of U20S cells using anti-GSTM1 Antibody. Overlay histogram showing U20S cells stained with anti-GSTM1 Antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-GSTM1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Monoclonal Mouse anti‑Human GSTM1 Antibody (IHC, WB) LS‑C756622

Monoclonal Mouse anti‑Human GSTM1 Antibody (IHC, WB) LS‑C756622

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Antibody:
GSTM1 Mouse anti-Human Monoclonal Antibody
Application:
IHC-P, IHC-Fr, ICC, WB, Flo
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C756622-10
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
GSTM1 Mouse anti-Human Monoclonal Antibody
Application:
IHC-P, IHC-Fr, ICC, WB, Flo
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
GSTM1 antibody LS-C756622 is an unconjugated mouse monoclonal antibody to GSTM1 from human. It is reactive with human, mouse and rat. Validated for Flow, ICC, IHC and WB.
Target
Human GSTM1
Synonyms
GSTM1 | Glutathione S-aryltransferase | GSTM1-1 | GSTM1a-1a | GSTM1b-1b | Glutathione S-alkyltransferase | GTH4 | GST class-mu 1 | GST1 | GTM1 | H-B | HB subunit 4 | MU-1 | Glutathione S-transferase M1 | Glutathione S-transferase mu 1 | GST HB subunit 4
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence of human GSTM1 (EEEKIRVDIL ENQTMDNHMQLGMICYNPEFEKLK).
Specificity
No cross reactivity with other proteins.
Applications
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • IHC - Frozen (0.5 - 1 µg/ml)
  • ICC (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
  • Flow Cytometry (1 - 3 µg/10E6 cells)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GSTM1
P09488 NM_000561 NP_000552.2

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Featured Products

GSTM1 Antibody - Anti-GSTM1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
GSTM1 Antibody (aa31‑80) LS‑B963
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
GSTM1 Antibody - Anti-GSTM1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IHC‑plus™ GSTM1 Antibody LS‑B4796
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
GSTM1 Antibody - IHC of paraffin-embedded liver cancer tissues using GSTM1 mouse monoclonal antibody with DAB staining.
GSTM1 Antibody (clone 1H4A4) LS‑C169444
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry, ELISA
GSTM1 Antibody - Western Blot of Mouse anti-GSTM1 Monoclonal Antibody. Lane 1: rGSTM1 protein. Lane 2: GST. Load: 50 ng per lane. Primary antibody: Mouse anti-GSTM1 Monoclonal Antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase conjugated Rb-a-Ms IgG at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 27 kDa, 27 kDa for GSTM1.
GSTM1 Antibody (clone 1H7.D8.G5) LS‑C744852
Species: Mouse, Human
Applications: IHC, Western blot, Immunoprecipitation, ELISA
GSTM1 ELISA Kit
Rat GSTM1 (Sandwich ELISA) ELISA Kit - LS-F25220
Reactivity: Rat
Range: 6.25-400 ng/ml
GSTM1 Protein - Recombinant Glutathione S Transferase Mu 1 By SDS-PAGE
Chicken GSTM1 Protein (Recombinant His, N-Terminal) (aa1-220) - LS-G12575
Source: E. coli
Tag: His, N-Terminal
See all GSTM1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/23/2024
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Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Tel: (800) 227-6666 / (650) 697-3600

To place an order:
customerservice@vectorlabs.com

For questions about orders:
Customer.Support@LSBio.com

For technical assistance:
Technical.Support@LSBio.com