Target
Human GRM5 / MGLUR5
Synonyms
GRM5 | GPRC1E | MGLUR5 | Mglur5b | MGlu5
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
NS0-derived rhmGluR5. (aa 19 - 509).
Specificity
Recognizes human mGluR5.
Applications
- Western blot (1 - 2 µg/ml)
- Flow Cytometry (25 µg/ml)
- ELISA (0.5 - 1 µg/ml)
Usage
Suitable for use in Flow Cytometry, ELISA and Western Blot. Intracellular Flow Cytometry: 25 ug/ml; Use 10 ul to label 1-5x10^5 cells in a total reaction volume not exceeding 200ul. Cells must be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin in PBS. Direct ELISA: 0.5-1.0 ug/ml; The detection limit is ~2 ng/well. Western Blot: 1-2 ug/ml; The detection limit is ~25 ng/lane under non-reducing and reducing conditions. Positive control: Flow Cytometry: MG-63 cells.
Presentation
Lyophilized from PBS, 5% Trehalose
Reconstitution
Reconstitute in 200 ul sterile PBS
Storage
Store at -20°C to -70°C for up to 1 year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee