Target
Rat GRIN1 / NMDAR1
Synonyms
GRIN1 | GluN1 | NMDAR1 | NR1 | MRD8 | NMD-R1 | NMDA receptor A1 | NMDA1 | NR1a | GluR zeta 1 | N-methyl-D aspartate receptor
Reactivity
Rat, Human, Monkey, Mouse, Hamster
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Tissue culture supernatant
Immunogen
Synthetic peptide LQNQKDTVLPRRAIEREEGQLQLCSRHRES corresponding to aa909-938 from the C-Terminus of rat NMDA receptor subunit. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster (100%); Panda, Dog, Horse, Opossum (93%).
Specificity
Recognizes rat splice variants NR1-1a, NR1-1b, NR1-2a, NR1-2b at ~120kD. These appear to be the major splice variants expressed in rat brain. Reactivity in mouse is weak. No cross-reaction with other glutamate receptor subunits. Species sequence homology: monkey, gerbil, rabbit, feline and fish.
Applications
- IHC (1:1000 - 1:2000)
- Western blot (1:1000 - 1:2000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for use in Western blot and Immunohistochemistry. Western Blot: 1:1000-1:2000 using ECL on rat brain lysate. Labels a single band at approximately 120kD corresponding to NMDAR1 in rat brain lysate. Immunohistochemistry: 1:1000-1:2000 with light microscopy. Tested with 4% paraformaldehyde or paraformaldehyde/glutaraldehyde fixed rat tissue.
Presentation
0.05% Sodium Azide
Storage
May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C. Aliquots are stable for up to 1 year at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee