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Catalog Number Size Price
LS-C796973-100 100 µg $539 
GPR49 / LGR5 Antibody - Immunohistochemical staining of paraffin-embedded human gastric cancer using mouse anti-LGR5 clone UMAB212  at 1:400 with GBI Polink2 Broad HRP DAB detection kit. (D22-110); pretreatment of tissue prior to stain with heat-induced epitope retrieval buffer from GBI Labs TEE pH 9.0. (B21-100) using pressure chamber for 3 minutes at 110C is required for optimal staining. Shown here strong cytoplamic tumor cells.
GPR49 / LGR5 Antibody - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 110C for 10 min. (1:150)
GPR49 / LGR5 Antibody - Immunohistochemical staining of paraffin-embedded human small intestine using anti-LGR5 clone UMAB212 mouse monoclonal antibody used at 1ug/mL dilution and detection with Polink2 Broad HRP DAB.requires heat-induced epitope retrieval with TEE pH 9.0 at 95-100C 20 minutes. The image shows two arrows pointing to strong and weak staining of small intestine stem cells.
GPR49 / LGR5 Antibody - Immunohistochemical staining of paraffin-embedded human colon cancer using mouse anti-LGR5 clone UMAB212  at 1:400 with GBI Polink2 Broad HRP DAB detection kit. (D22-110); pretreatment of tissue prior to stain with heat-induced epitope retrieval buffer from GBI Labs TEE pH 9.0. (B21-100)using pressure chamber for 3 minutes at 110C is required for optimal staining. Shown here strong cytoplamic tumor cells.
GPR49 / LGR5 Antibody - Anti-LGR5 mouse monoclonal antibody  immunofluorescent staining of MCF7 cells transiently transfected by pCMV6-ENTRY mouse LGR5. (1:100)
GPR49 / LGR5 Antibody - Anti-LGR5 mouse monoclonal antibody immunofluorescent staining of MCF7 cells transiently transfected by pCMV6-ENTRY human LGR5. (1:100)
GPR49 / LGR5 Antibody - Western blot analysis of extracts. (15ug) from 2 Human tissue by using anti-LGR5 monoclonal antibody(left lane: Brain right lane: Testis). (1:2000)
GPR49 / LGR5 Antibody - Western blot analysis of extracts. (10ug) from 7 different transiently transfected HEK293T cell lysates by using anti-LGR5 monoclonal antibody(1:2000). (Lanes: control vector pCMV6-Entry ; human LGR4 , LGR5  and LGR6 ; or mouse LGR4,LGR5  and LGR6).
GPR49 / LGR5 Antibody - NIH-3T3. (Left lane) or stable expressed LGR5-3T3. (Right lane) cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGR5. (1:1000)
GPR49 / LGR5 Antibody - Flow cytometric analysis of HEK293T cells transiently transfected with human LGR4  or mouse LGR4 , human LGR5  or mouse LGR5 , human LGR6  or mouse LGR6 , or control vector pCMV6-Entry , using anti-LGR5 antibody 1:100) surface stained on live cells.
GPR49 / LGR5 Antibody - Flow cytometric analysis of stable expression LGR5 HEK293T cells. (Red) compared to negative control HEK293T cells. (Blue) using anti-LGR5 antibody 1:100) surface stained on live cells.
GPR49 / LGR5 Antibody - OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-LGR5 mouse monoclonal antibody  at 1:100. The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
GPR49 / LGR5 Antibody - Immunohistochemical staining of paraffin-embedded human gastric cancer using mouse anti-LGR5 clone UMAB212  at 1:400 with GBI Polink2 Broad HRP DAB detection kit. (D22-110); pretreatment of tissue prior to stain with heat-induced epitope retrieval buffer from GBI Labs TEE pH 9.0. (B21-100) using pressure chamber for 3 minutes at 110C is required for optimal staining. Shown here strong cytoplamic tumor cells.
GPR49 / LGR5 Antibody - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer. (pH8.5) at 110C for 10 min. (1:150)
GPR49 / LGR5 Antibody - Immunohistochemical staining of paraffin-embedded human small intestine using anti-LGR5 clone UMAB212 mouse monoclonal antibody used at 1ug/mL dilution and detection with Polink2 Broad HRP DAB.requires heat-induced epitope retrieval with TEE pH 9.0 at 95-100C 20 minutes. The image shows two arrows pointing to strong and weak staining of small intestine stem cells.
GPR49 / LGR5 Antibody - Immunohistochemical staining of paraffin-embedded human colon cancer using mouse anti-LGR5 clone UMAB212  at 1:400 with GBI Polink2 Broad HRP DAB detection kit. (D22-110); pretreatment of tissue prior to stain with heat-induced epitope retrieval buffer from GBI Labs TEE pH 9.0. (B21-100)using pressure chamber for 3 minutes at 110C is required for optimal staining. Shown here strong cytoplamic tumor cells.
GPR49 / LGR5 Antibody - Anti-LGR5 mouse monoclonal antibody  immunofluorescent staining of MCF7 cells transiently transfected by pCMV6-ENTRY mouse LGR5. (1:100)
GPR49 / LGR5 Antibody - Anti-LGR5 mouse monoclonal antibody immunofluorescent staining of MCF7 cells transiently transfected by pCMV6-ENTRY human LGR5. (1:100)
GPR49 / LGR5 Antibody - Western blot analysis of extracts. (15ug) from 2 Human tissue by using anti-LGR5 monoclonal antibody(left lane: Brain right lane: Testis). (1:2000)
GPR49 / LGR5 Antibody - Western blot analysis of extracts. (10ug) from 7 different transiently transfected HEK293T cell lysates by using anti-LGR5 monoclonal antibody(1:2000). (Lanes: control vector pCMV6-Entry ; human LGR4 , LGR5  and LGR6 ; or mouse LGR4,LGR5  and LGR6).
GPR49 / LGR5 Antibody - NIH-3T3. (Left lane) or stable expressed LGR5-3T3. (Right lane) cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGR5. (1:1000)
GPR49 / LGR5 Antibody - Flow cytometric analysis of HEK293T cells transiently transfected with human LGR4  or mouse LGR4 , human LGR5  or mouse LGR5 , human LGR6  or mouse LGR6 , or control vector pCMV6-Entry , using anti-LGR5 antibody 1:100) surface stained on live cells.
GPR49 / LGR5 Antibody - Flow cytometric analysis of stable expression LGR5 HEK293T cells. (Red) compared to negative control HEK293T cells. (Blue) using anti-LGR5 antibody 1:100) surface stained on live cells.
GPR49 / LGR5 Antibody - OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-LGR5 mouse monoclonal antibody  at 1:100. The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
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Monoclonal Mouse anti‑Human GPR49 / LGR5 Antibody (clone UMAB212, Carrier‑free, aa22‑543, IHC, IF, WB) LS‑C796973

Monoclonal Mouse anti‑Human GPR49 / LGR5 Antibody (clone UMAB212, Carrier‑free, aa22‑543, IHC, IF, WB) LS‑C796973

Antibody:
GPR49 / LGR5 Mouse anti-Human Monoclonal (aa22-543) (Carrier-free) (UMAB212) Antibody
Application:
IHC, IF, WB, Flo, PMA
Reactivity:
Human
Format:
Unconjugated, Carrier-free
Other formats:
Price
Catalog Number
$539
LS-C796973-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
GPR49 / LGR5 Mouse anti-Human Monoclonal (aa22-543) (Carrier-free) (UMAB212) Antibody
Application:
IHC, IF, WB, Flo, PMA
Reactivity:
Human
Format:
Unconjugated, Carrier-free
Other formats:

Specifications

Description
LGR5 antibody LS-C796973 is an unconjugated mouse monoclonal antibody to human LGR5 (GPR49) (aa22-543). Validated for Flow, IF, IHC, PMA and WB.
Target
Human GPR49 / LGR5
Synonyms
LGR5 | FEX | G-protein coupled receptor 49 | G protein-coupled receptor 49 | GPR67 | GRP49 | GPR49 | HG38 | G-protein coupled receptor 67
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Clone
UMAB212
Conjugations
Unconjugated
Purification
Affinity chromatography
Modifications
Carrier-free. Also available Unmodified.
Immunogen
Human recombinant protein fragment corresponding to amino acids 22-543 of human LGR5 (NP_003658) produced in HEK293 cell.
Epitope
aa22-543
Specificity
The specificity of this antibody has been validated by testing against a high-density Protein Microarray containing more than 17,000 recombinant proteins.
Applications
  • IHC (1:100 - 1:150)
  • Immunofluorescence (1:100)
  • Western blot (1:1000 - 1:2000)
  • Flow Cytometry (1:100)
  • Protein Microarray
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.3, 8% Trehalose
Reconstitution
Reconstitute with PBS pH 7.3. To use this carrier-free antibody for conjugation experiment, we strongly recommend you to perform another round of desalting process.
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GPR49 / LGR5
O75473 NM_003667 NP_003658.1

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Requested From: United States
Date Requested: 12/13/2024