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Catalog Number Size Price
LS-C209440-1 1 mg (1 mg/ml) $376 
Mouse IgG2a Antibody - ELISA results of purified Goat anti-Mouse IgG2a Antibody Peroxidase conjugated tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 1.0 µg of Mouse IgG2a. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate

Polyclonal Goat Anti‑Mouse IgG2a Secondary Antibody (HRP, IHC, WB) LS‑C209440

Polyclonal Goat Anti‑Mouse IgG2a Secondary Antibody (HRP, IHC, WB) LS‑C209440

Antibody:
Mouse IgG2a Goat anti-Mouse Polyclonal (HRP) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Mouse
Format:
Horseradish Peroxidase, Unmodified
Price
Catalog Number
$376
LS-C209440-1
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Mouse IgG2a Goat anti-Mouse Polyclonal (HRP) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Mouse
Format:
Horseradish Peroxidase, Unmodified

Specifications

Description
Mouse IgG2a antibody LS-C209440 is an HRP-conjugated goat polyclonal antibody. Validated for ELISA, IHC and WB.
Host
Goat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Horseradish Peroxidase
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Mouse IgG2a heavy chain.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-Goat Serum, Mouse Serum and Mouse IgG2a. Specificity was confirmed by ELISA at less than 1% cross-reactivity against other mouse heavy or light chain isotypes. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
Applications
  • IHC (1:1000 - 1:5000)
  • Western blot (1:2000 - 1:10000)
  • ELISA (1:20000 - 1:100000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Anti-Mouse IgG2a Peroxidase Secondary antibodies are suitable for ELISA, Immunohistochemistry, western blotting as well as other anti IgG2a antibody based assays. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Reconstitution
Reconstitute in 1 mL of deionized water.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 7/17/2024