Mouse IgG2a

Goat

Mouse (tested or 100% immunogen sequence identity)

IgG Polyclonal

Atto 425

Affinity chromatography

Unmodified

Mouse IgG2a heavy chain.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG2a. Specificity was confirmed by ELISA at less than 1% cross-reactivity against other mouse heavy or light chain isotypes. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.

• Immunofluorescence (1:5000)
• Western blot (1:4000 - 1:20000)
• Flow Cytometry (1:500 - 1:2500)
• Fluorophore-Linked Immunosorbent Assay (1:20000)

Anti-Mouse IgG2a secondary antibody is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG2a antibody based assays. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Reconstitute in 500 µL of deionized water.

Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.