Human Kappa Light Chain

Goat

Human (tested or 100% immunogen sequence identity)

IgG F(ab')2 Polyclonal

Horseradish Peroxidase

Affinity chromatography

Unmodified

Human kappa light chain.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.

• IHC (1:1000 - 1:5000)
• Western blot (1:2000 - 1:10000)
• ELISA (1:50000)

Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature.

Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.125 M Sodium Borate, pH 8.0, 0.005 M EDTA, 0.075 M NaCl, 0.01% Thimerosal

Reconstitute in 1 mL of deionized water.

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.