Target
Human Kappa Light Chain
Reactivity
Human
(tested or 100% immunogen sequence identity)
Purification
Affinity chromatography
Immunogen
Human kappa light chain.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum. Specificity was confirmed by ELISA minimal cross-reactivity against other human heavy or light chain isotypes.
Applications
- Immunofluorescence (1:1000 - 1:5000)
- Flow Cytometry (1:500 - 1:2500)
- Fluorophore-Linked Immunosorbent Assay (1:10000 - 1:50000)
Usage
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody- based fluorescent assays requiring lot-to-lot consistency. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.125 M Sodium Borate, pH 8.0, 0.005 M EDTA, 0.075 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 1 mL of deionized water.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee