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Catalog Number Size Price
LS-C61385-1 1 mg $417 
Human IgG Fab'2 Antibody - ELISA results of purified Goat F(ab')2 Anti-HUMAN IgG F(ab')2 Biotin Conjugated Antibody (Min X Bv Hs Ms & Rt Serum Proteins) tested against purified HUMAN IgG F(ab')2 . Each well was coated in duplicate with 1.0 µg of HUMAN IgG F(ab')2 (green line). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-Streptavidin HRP conjugate, and TMB-1000 substrate.

Polyclonal Goat Anti‑Human IgG Fab'2 Secondary Antibody (Biotin, IHC, WB) LS‑C61385

Polyclonal Goat Anti‑Human IgG Fab'2 Secondary Antibody (Biotin, IHC, WB) LS‑C61385

Antibody:
Human IgG Fab'2 Goat anti-Human Polyclonal (Biotin) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Biotin, Unmodified
Price
Catalog Number
$417
LS-C61385-1
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
Human IgG Fab'2 Goat anti-Human Polyclonal (Biotin) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Biotin, Unmodified

Specifications

Description
Human IgG Fab'2 antibody LS-C61385 is a biotin-conjugated goat polyclonal antibody. Validated for ELISA, IHC and WB.
Target
Human IgG Fab'2
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG F(ab')2 Polyclonal
Conjugations
Biotin
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Human IgG F(ab')2 fragment
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Human IgG, Human IgG F(ab’)2 and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG F(c) or Bovine, Horse, Mouse, and Rat Serum Proteins.
Applications
  • IHC (1:1000 - 1:5000)
  • Western blot (1:5000 - 1:40000)
  • ELISA (1:500000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 1 mL of deionized water.
Storage
Store at 4°C while lyophilized, once reconstituted aliquot and freeze at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/26/2024