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Catalog Number	Size	Price
LS-C800259-100	100 µl (1 mg/ml)	$379
LS-C800259-200	200 µl (1 mg/ml)	$421

GJA1 / CX43 / Connexin 43 Antibody - 1:200 staining human kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

GJA1 / CX43 / Connexin 43 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.

GJA1 / CX43 / Connexin 43 Antibody - Western blot analysis on HuvEc cell lysates using Connexin 43 antibody. The lane on the left is treated with the antigen-specific peptide.

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Polyclonal Rabbit anti‑Human GJA1 / CX43 / Connexin 43 Antibody (IHC, IF, WB) LS‑C800259

Name: Anti-GJA1 / CX43 / Connexin 43 Antibody | Rabbit anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-C800259-100
Price: 379.0000 USD
Availability: InStock

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Antibody:

GJA1 / CX43 / Connexin 43 Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IF, WB, Peptide-ELISA

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

$379

LS-C800259-100

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

GJA1 / CX43 / Connexin 43 Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IF, WB, Peptide-ELISA

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

Connexin 43 antibody LS-C800259 is an unconjugated rabbit polyclonal antibody to Connexin 43 (GJA1 / CX43) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.

Target

Human GJA1 / CX43 / Connexin 43

Synonyms

See All GJA1 / CX43 / Connexin 43 Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

IgG Polyclonal

Conjugations

Unconjugated

Purification

Peptide affinity chromatography.

Modifications

Unmodified

Immunogen

Synthesized peptide derived from human Connexin 43

Specificity

Connexin 43 antibody detects endogenous levels of total Connexin 43.

Applications

IHC (1:50 - 1:200)
Immunofluorescence
Western blot (1:500 - 1:3000)
Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.

Presentation

PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol

Storage

Upon receipt, store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

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About GJA1 / CX43 / Connexin 43