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Catalog Number Size Price
LS-C343860-10 10 µg $318 
LS-C343860-100 100 µg (0.5 mg/ml) $470 
GJA1 / CX43 / Connexin 43 Antibody - IHC analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody. Connexin 43/GJA1 was detected in paraffin-embedded section of mouse cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Connexin 43/GJA1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
GJA1 / CX43 / Connexin 43 Antibody - IHC analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody. Connexin 43/GJA1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Connexin 43/GJA1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
GJA1 / CX43 / Connexin 43 Antibody - IHC analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody. Connexin 43/GJA1 was detected in paraffin-embedded section of rat cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Connexin 43/GJA1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
GJA1 / CX43 / Connexin 43 Antibody - IHC analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody. Connexin 43/GJA1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Connexin 43/GJA1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
GJA1 / CX43 / Connexin 43 Antibody - Connexin 43/GJA1 antibody IHC-paraffin: Human Lung Cancer Tissue.
GJA1 / CX43 / Connexin 43 Antibody - Connexin 43/GJA1 antibody IHC-paraffin: Rat Cardiac Muscle Tissue.
GJA1 / CX43 / Connexin 43 Antibody - Connexin 43/GJA1 antibody IHC-frozen: Rat Cardiac Muscle Tissue.
GJA1 / CX43 / Connexin 43 Antibody - Western blot analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: rat hear tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Connexin 43/GJA1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Connexin 43/GJA1 at approximately 43KD. The expected band size for Connexin 43/GJA1 is at 43KD.
GJA1 / CX43 / Connexin 43 Antibody - Connexin 43/GJA1 antibody Western blot. WB: Rat Heart Tissue Lysate.
GJA1 / CX43 / Connexin 43 Antibody - IHC analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody. Connexin 43/GJA1 was detected in paraffin-embedded section of mouse cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Connexin 43/GJA1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
GJA1 / CX43 / Connexin 43 Antibody - IHC analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody. Connexin 43/GJA1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Connexin 43/GJA1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
GJA1 / CX43 / Connexin 43 Antibody - IHC analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody. Connexin 43/GJA1 was detected in paraffin-embedded section of rat cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Connexin 43/GJA1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
GJA1 / CX43 / Connexin 43 Antibody - IHC analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody. Connexin 43/GJA1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Connexin 43/GJA1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
GJA1 / CX43 / Connexin 43 Antibody - Connexin 43/GJA1 antibody IHC-paraffin: Human Lung Cancer Tissue.
GJA1 / CX43 / Connexin 43 Antibody - Connexin 43/GJA1 antibody IHC-paraffin: Rat Cardiac Muscle Tissue.
GJA1 / CX43 / Connexin 43 Antibody - Connexin 43/GJA1 antibody IHC-frozen: Rat Cardiac Muscle Tissue.
GJA1 / CX43 / Connexin 43 Antibody - Western blot analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: rat hear tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Connexin 43/GJA1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Connexin 43/GJA1 at approximately 43KD. The expected band size for Connexin 43/GJA1 is at 43KD.
GJA1 / CX43 / Connexin 43 Antibody - Connexin 43/GJA1 antibody Western blot. WB: Rat Heart Tissue Lysate.
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Polyclonal Rabbit anti‑Human GJA1 / CX43 / Connexin 43 Antibody (aa351‑367, IHC, WB) LS‑C343860

Polyclonal Rabbit anti‑Human GJA1 / CX43 / Connexin 43 Antibody (aa351‑367, IHC, WB) LS‑C343860

Note: This antibody replaces LS-C388289
Antibody:
GJA1 / CX43 / Connexin 43 Rabbit anti-Human Polyclonal (aa351-367) Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C343860-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
GJA1 / CX43 / Connexin 43 Rabbit anti-Human Polyclonal (aa351-367) Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Connexin 43 antibody LS-C343860 is an unconjugated rabbit polyclonal antibody to Connexin 43 (GJA1 / CX43) (aa351-367) from human. It is reactive with human, mouse and rat. Validated for ICC, IHC and WB.
Target
Human GJA1 / CX43 / Connexin 43
Synonyms
GJA1 | Connexin-43 | DFNB38 | Connexin 43 | Gap junction alpha-1 protein | GJAL | HSS | Gap junction protein alpha 1 | ODDD | ODD | AVSD3 | CX43 | HLHS1 | ODOD | SDTY3
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human Connexin 43(351-367 aa HELQPLAIVDQRPSSRA), identical to the related rat and mouse sequences.
Epitope
aa351-367
Specificity
Expressed in the heart and fetal cochlea. .
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • ICC
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GJA1 / CX43 / Connexin 43
P17302 NM_000165 NP_000156.1

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 11/26/2024