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Antibodies > GFP > LS-C154186
 
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Catalog Number Size Price
LS-C154186-1 1 mg $768 
GFP Antibody - Anti-GFP Antibody - Immunofluorescence Microscopy. polyclonal anti-GFP antibody at a 1:1000 dilution detects tau-GFP in cell bodies (large arrowhead) and axons of motorneurons (arrow) and interneurons (small arrowhead) in Drosophila melanogaster late stage embryonic central nervous system. Fluorochrome conjugated anti-Goat secondary antibody was used for detection at 1:300. Panel A shows a lateral view (ventral left) and Panels B and C show ventral views of whole mount embryos at 63x magnification (plus 2x digital zoom). In all panels, anterior is up. Personal Communication, Helmata Mistry, Washington University School of Medicine, St. Louis, MO. This image was taken for the unconjugated form of this product. Other forms have not been tested.
GFP Antibody - Anti-GFP Antibody - Western Blot. Western blot of GFP recombinant protein detected with polyclonal anti-GFP antibody. Lane 1 shows detection of a 33 kD band corresponding to a GFP containing recombinant protein (arrowhead) expressed in HeLa cells. Lane 2 shows no staining of a mock transfected HeLa cell lysate. A 4-12% Bis-Tris gradient gel was used for SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody diluted to 1 ug/ml for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye 800 conjugated Donkey-a-Goat IgG [H&L] MX7 (. The IRDye 800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. This image was taken for the unconjugated form of this product. Other forms have not been tested.
GFP Antibody - Anti-GFP Antibody - Immunofluorescence Microscopy. polyclonal anti-GFP antibody at a 1:1000 dilution detects tau-GFP in cell bodies (large arrowhead) and axons of motorneurons (arrow) and interneurons (small arrowhead) in Drosophila melanogaster late stage embryonic central nervous system. Fluorochrome conjugated anti-Goat secondary antibody was used for detection at 1:300. Panel A shows a lateral view (ventral left) and Panels B and C show ventral views of whole mount embryos at 63x magnification (plus 2x digital zoom). In all panels, anterior is up. Personal Communication, Helmata Mistry, Washington University School of Medicine, St. Louis, MO. This image was taken for the unconjugated form of this product. Other forms have not been tested.
GFP Antibody - Anti-GFP Antibody - Western Blot. Western blot of GFP recombinant protein detected with polyclonal anti-GFP antibody. Lane 1 shows detection of a 33 kD band corresponding to a GFP containing recombinant protein (arrowhead) expressed in HeLa cells. Lane 2 shows no staining of a mock transfected HeLa cell lysate. A 4-12% Bis-Tris gradient gel was used for SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody diluted to 1 ug/ml for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye 800 conjugated Donkey-a-Goat IgG [H&L] MX7 (. The IRDye 800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Polyclonal Goat anti‑Aequorea victoria GFP Antibody (FITC, IF, WB) LS‑C154186

Polyclonal Goat anti‑Aequorea victoria GFP Antibody (FITC, IF, WB) LS‑C154186

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Antibody:
GFP Goat anti-Aequorea victoria Polyclonal (FITC) Antibody
Application:
IF, WB, FLISA
Reactivity:
Aequorea victoria
Format:
FITC, Unmodified
Price
Catalog Number
$768
LS-C154186-1
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
GFP Goat anti-Aequorea victoria Polyclonal (FITC) Antibody
Application:
IF, WB, FLISA
Reactivity:
Aequorea victoria
Format:
FITC, Unmodified

Specifications

Description
GFP antibody LS-C154186 is an FITC-conjugated goat polyclonal antibody to aequorea victoria GFP. Validated for FLISA, IF and WB.
Target
Aequorea victoria GFP
Synonyms
GFP
Host
Goat
Reactivity
Aequorea victoria (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
FITC
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Fluorescein and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse and Rat Serum Proteins.
Applications
  • Immunofluorescence (1:500 - 1:2500)
  • Western blot (1:10000)
  • Fluorophore-Linked Immunosorbent Assay (1:20000)
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 1 mL of deionized water.
Storage
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted. Protect from light.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GFP
P42212 M62653 P42212

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Featured Products

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Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
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Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, ELISA
GFP Antibody - Anti-GFP Chemiluminescent Kit for Western blot. Known amounts of recombinant GFP protein ( were spiked into a HeLa cell-derived lysates (p/n W09-000-364) and separated by SDS-PAGE using a 4-20% gradient gel. Proteins were transferred onto a nitrocellulose membrane for 1 h at 100 mV. The membrane was blocked with TTBS (p/n MB-013) supplemented with 1% BSA (p/n BSA-50) for 1 h at 4? prior to probing the blot with the anti-GFP monoclonal antibody Anti-GFP (MOUSE) Monoclonal Antibody - 600-301-215 diluted 1:1000 for 40 min. Detection of the primary antibody by the HRP-conjugated anti-Mouse IgG (p/n LS-C60772) was performed at a dilution of 1:20000 for 1h at 4?. FemtoMax Super Sensitive Chemiluminescent Luminol Substrate was used for signal detection (see below).
GFP Antibody (clone 9F9.F9) LS‑C154208
Species: Aequorea victoria
Applications: IHC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
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GFP Antibody (HRP) LS‑C50850
Species: Aequorea victoria
Applications: IHC, Immunofluorescence, Western blot, ELISA
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GFP Antibody (Biotin) LS‑C50849
Species: Aequorea victoria
Applications: IHC, Western blot, ELISA
See all GFP Antibodies

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/26/2024
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