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Antibodies > GFP > LS-C46139
 
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Catalog Number Size Price
LS-C46139-0.1 0.1 mg (1 mg/ml) $316 
GFP Antibody - Confocal microscopy images of COS-7 cells transfected with expression constructs encoding membrane-tethered EGFP (membrane-EGFP; top) or nuclear Polycomb 2-EYFP fusion protein (Pc2-EYFP; bottom). The natural fluorescence of the produced proteins is shown in the green channel (left), polyclonal anti-GFP antibody signal was detected in the red channel (right). The system was carefully tested for overlap of these two optical channels and images were scanned separately in sequential scanning mode. The blue nuclear stain is also shown.
GFP Antibody - Immunoprecipitation of GFP-NLS from HEK293 cells using anti-GFP antibody. HEK293 cells were transfected with expression construct encoding GFP-NLS protein. Twenty hours post transfection cells were lysed in non-denaturating conditions (Lysis buffer: 20 mM Tris, pH 7.5, 100 mM NaCl, 0.5% Triton X-100, inhibitors of proteases). Aliquots of cell lysate were immunoprecipitated using a polyclonal anti-GFP antibody (lane 2) or a pre-immune rabbit serum (lane 3). Immunoprecipitates together with a sample of the cell lysate (lane 1) were separated on SDS-PAGE polyacrylamide gel and immunoblotted with the anti-GFP antibody. The positions of molecular weight markers in kDa are indicated at the left.
GFP Antibody - Confocal microscopy images of COS-7 cells transfected with expression constructs encoding membrane-tethered EGFP (membrane-EGFP; top) or nuclear Polycomb 2-EYFP fusion protein (Pc2-EYFP; bottom). The natural fluorescence of the produced proteins is shown in the green channel (left), polyclonal anti-GFP antibody signal was detected in the red channel (right). The system was carefully tested for overlap of these two optical channels and images were scanned separately in sequential scanning mode. The blue nuclear stain is also shown.
GFP Antibody - Immunoprecipitation of GFP-NLS from HEK293 cells using anti-GFP antibody. HEK293 cells were transfected with expression construct encoding GFP-NLS protein. Twenty hours post transfection cells were lysed in non-denaturating conditions (Lysis buffer: 20 mM Tris, pH 7.5, 100 mM NaCl, 0.5% Triton X-100, inhibitors of proteases). Aliquots of cell lysate were immunoprecipitated using a polyclonal anti-GFP antibody (lane 2) or a pre-immune rabbit serum (lane 3). Immunoprecipitates together with a sample of the cell lysate (lane 1) were separated on SDS-PAGE polyacrylamide gel and immunoblotted with the anti-GFP antibody. The positions of molecular weight markers in kDa are indicated at the left.
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Polyclonal Rabbit anti‑Aequorea victoria GFP Antibody (clone Polyclonal, WB) LS‑C46139

Polyclonal Rabbit anti‑Aequorea victoria GFP Antibody (clone Polyclonal, WB) LS‑C46139

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Antibody:
GFP Rabbit anti-Aequorea victoria Polyclonal (Polyclonal) Antibody
Application:
ICC, WB, IP
Reactivity:
Aequorea victoria, All species
Format:
Unconjugated, Unmodified
Price
Catalog Number
$316
LS-C46139-0.1
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
GFP Rabbit anti-Aequorea victoria Polyclonal (Polyclonal) Antibody
Application:
ICC, WB, IP
Reactivity:
Aequorea victoria, All species
Format:
Unconjugated, Unmodified

Specifications

Description
GFP antibody LS-C46139 is an unconjugated rabbit polyclonal antibody to all species GFP. Validated for ICC, IP and WB.
Target
Aequorea victoria GFP
Synonyms
GFP
Host
Rabbit
Reactivity
Aequorea victoria, All species
Clonality
Polyclonal
Clone
Polyclonal
Conjugations
Unconjugated
Purification
Purified from rabbit serum by affinity chromatography. Greater than 95% by SDS-PAGE.
Modifications
Unmodified
Immunogen
EGFP, a native full-length protein.
Specificity
The polyclonal antibody recognizes GFP, EGFP, EYFP fusion proteins in all species.
Applications
  • ICC
  • Western blot (0.5 - 1.5 µg/ml)
  • Immunoprecipitation
Presentation
PBS, pH 7.4, 15 mM Sodium Azide
Storage
Store at 2°C to 8°C. Do not freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GFP
P42212 M62653 P42212

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GFP Antibody - Anti-GFP Chemiluminescent Kit for Western blot. Known amounts of recombinant GFP protein ( were spiked into a HeLa cell-derived lysates (p/n W09-000-364) and separated by SDS-PAGE using a 4-20% gradient gel. Proteins were transferred onto a nitrocellulose membrane for 1 h at 100 mV. The membrane was blocked with TTBS (p/n MB-013) supplemented with 1% BSA (p/n BSA-50) for 1 h at 4? prior to probing the blot with the anti-GFP monoclonal antibody Anti-GFP (MOUSE) Monoclonal Antibody - 600-301-215 diluted 1:1000 for 40 min. Detection of the primary antibody by the HRP-conjugated anti-Mouse IgG (p/n LS-C60772) was performed at a dilution of 1:20000 for 1h at 4?. FemtoMax Super Sensitive Chemiluminescent Luminol Substrate was used for signal detection (see below).
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Applications: IHC, Western blot, ELISA
See all GFP Antibodies

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/26/2024
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