Target
Aequorea victoria GFP
Reactivity
Aequorea victoria
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Horseradish Peroxidase.
Also available conjugated with
AP,
TR,
Biotin,
FITC,
AP,
TR,
Biotin,
APC.
Purification
Protein A purified
Immunogen
GST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Specificity
Recognizes wild type and recombinant Green Fluorescent Protein. Does not recognize enhanced forms of GFP. Expected to detect GFP containing inserts by Immunofluorescence Microscopy on prokaryotic (E. coli) and eukaryotic (CHO cells) expression systems. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, anti-peroxidase, and purified and partially purified GFP (Aequorea victoria) serum.
Applications
- IHC (1:500 - 1:2500)
- Western blot (1:1000 - 1:5000)
- ELISA (1:10000 - 1:50000)
- Applications tested for the base form of this product only
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
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Usage
Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA (sandwich or capture): 1:10000-1:50000. Western Blot: 1:1000-1:5000. Immunohistochemistry: 1:500-1:2500. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
10 mg/ml BSA (IgG, Protease free), PBS, pH 7.2, 0.01% Thimerosal, 40% Glycerol
Storage
Short term: 4°C; Long term: -70°C; Protect from light.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee