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Catalog Number Size Price
LS-C776043-100 100 µg (1 mg/ml) $442 
GAPDH Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GAPDH Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GAPDH Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Nucleus, Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GAPDH Antibody. (D) Composite.
GAPDH Antibody - Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~36 kDa GAPDH protein using Rabbit Anti-GAPDH Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-GAPDH Polyclonal Antibody  at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~36 kDa.
GAPDH Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GAPDH Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GAPDH Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Nucleus, Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GAPDH Antibody. (D) Composite.
GAPDH Antibody - Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~36 kDa GAPDH protein using Rabbit Anti-GAPDH Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-GAPDH Polyclonal Antibody  at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~36 kDa.
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Polyclonal Rabbit anti‑Human GAPDH Antibody (IF, WB) LS‑C776043

Polyclonal Rabbit anti‑Human GAPDH Antibody (IF, WB) LS‑C776043

Antibody:
GAPDH Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$442
LS-C776043-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
GAPDH Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
GAPDH antibody LS-C776043 is an unconjugated rabbit polyclonal antibody to human GAPDH. Validated for IF and WB.
Target
Human GAPDH
Synonyms
GAPDH | A1 40 kd subunit | Activator 1 40 kd subunit | G3PD | GAPD | G3pdh | Rfc40 | Rf-c 40 kd subunit
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Peptide Affinity Purified
Modifications
Unmodified
Immunogen
Synthetic peptide from the mid-protein of Human GAPDH
Specificity
Detects ~36 kDa.
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
Usage
Applications should be user optimized.
Presentation
PBS, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GAPDH
P04406 NM_002046 NP_002037.2

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Requested From: United States
Date Requested: 11/23/2024