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Vector Laboratories, Inc.
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Customer Service: (800) 227-6666 / (650) 697-3600


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Catalog Number Size Price
LS-C20161-100 100 µg (0.5 mg/ml) $503 
GAPDH Antibody - GAPDH (Internal) Antibody (2µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
GAPDH Antibody - GAPDH (Internal) Antibody Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
GAPDH Antibody - GAPDH antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
GAPDH Antibody - GAPDH antibody (0.1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
GAPDH Antibody - GAPDH antibody (0.1µg/ml) staining of Mouse Liver (A) and (0.03ug/ml) Rat Heart (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
GAPDH Antibody - GAPDH antibody (0.1µg/ml) staining of Human Liver (A), (0.03ug/ml) Testes (B) and Tonsil (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
GAPDH Antibody - (0.1 ug/ml) staining of Human Tonsil Lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
GAPDH Antibody - GAPDH antibody (0.03µg/ml) staining of HeLa (A) and NIH3T3 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
GAPDH Antibody - GAPDH (Internal) Antibody (2µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
GAPDH Antibody - GAPDH (Internal) Antibody Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
GAPDH Antibody - GAPDH antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
GAPDH Antibody - GAPDH antibody (0.1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
GAPDH Antibody - GAPDH antibody (0.1µg/ml) staining of Mouse Liver (A) and (0.03ug/ml) Rat Heart (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
GAPDH Antibody - GAPDH antibody (0.1µg/ml) staining of Human Liver (A), (0.03ug/ml) Testes (B) and Tonsil (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
GAPDH Antibody - (0.1 ug/ml) staining of Human Tonsil Lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
GAPDH Antibody - GAPDH antibody (0.03µg/ml) staining of HeLa (A) and NIH3T3 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
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Polyclonal Goat anti‑Human GAPDH Antibody (aa92‑103, IHC, IF, WB) LS‑C20161

Polyclonal Goat anti‑Human GAPDH Antibody (aa92‑103, IHC, IF, WB) LS‑C20161

Note: This antibody replaces LS-C66763
Antibody:
GAPDH Goat anti-Human Polyclonal (aa92-103) Antibody
Application:
IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Dog, Hamster, Pig, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$503
LS-C20161-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
GAPDH Goat anti-Human Polyclonal (aa92-103) Antibody
Application:
IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Dog, Hamster, Pig, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
GAPDH antibody LS-C20161 is an unconjugated goat polyclonal antibody to GAPDH (aa92-103) from human. It is reactive with human, mouse, rat and other species. Validated for IF, IHC, Peptide-ELISA and WB. Cited in 3 publications.
Target
Human GAPDH
Synonyms
GAPDH | A1 40 kd subunit | Activator 1 40 kd subunit | G3PD | GAPD | G3pdh | Rfc40 | Rf-c 40 kd subunit
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Dog, Hamster, Pig, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-GVNHEKYDNSLK, from the internal region of the protein sequence according to NP_002037.2NP_001243728.1.
Epitope
aa92-103
Specificity
Human GAPDH. GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.
Applications
  • IHC - Paraffin (2 µg/ml)
  • Immunofluorescence (5 - 10 µg/ml)
  • Western blot (0.03 - 0.1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GAPDH
P04406 NM_002046 NP_002037.2

LSBio Ratings

GAPDH Antibody (aa92-103) for IHC, IF/Immunofluorescence, WB/Western LS-C20161 has an LSBio Rating of
Publications (4.2)

Publications (3)

Elucidating the phenomenon of HESC-derived RPE: anatomy of cell genesis, expansion and retinal transplantation. Vugler A, Carr AJ, Lawrence J, Chen LL, Burrell K, Wright A, Lundh P, Semo M, Ahmado A, Gias C, da Cruz L, Moore H, Andrews P, Walsh J, Coffey P. Experimental neurology. 2008 214:347-61.

Molecular characterization and functional analysis of phagocytosis by human embryonic stem cell-derived RPE cells using a novel human retinal assay. Carr AJ, Vugler A, Lawrence J, Chen LL, Ahmado A, Chen FK, Semo M, Gias C, da Cruz L, Moore HD, Walsh J, Coffey PJ. Molecular vision. 2009 15:283-95.

The expression of retinal cell markers in human retinal pigment epithelial cells and their augmentation by the synthetic retinoid fenretinide. Carr AJ, Vugler AA, Yu L, Semo M, Coffey P, Moss SE, Greenwood J. Molecular vision. 2011 17:1701-15.

Customer Reviews (0)


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Requested From: United States
Date Requested: 11/23/2024