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Catalog Number Size Price
LS-C97485-400 400 µl $393 
GAPDH Antibody - Formalin-fixed and paraffin-embedded human prostate carcinoma with GAPDH Antibody (C-term R248), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
GAPDH Antibody - Confocal immunofluorescence of GAPDH Antibody (C-term R248) with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
GAPDH Antibody - Confocal immunofluorescence of GAPDH Antibody (C-term R248) with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
GAPDH Antibody - Western blot of GAPDH Antibody (C-term R248) in A2058, A375, CEM cell line lysates (35 ug/lane). GAPDH (arrow) was detected using the purified antibody.
GAPDH Antibody - Western blot of lysates from HeLa,HUVEC cell line (from left to right),using GAPDH Antibody (C-term R248). Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
GAPDH Antibody - Flow cytometric of HepG2 cells using GAPDH Antibody (C-term R248)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
GAPDH Antibody - Formalin-fixed and paraffin-embedded human prostate carcinoma with GAPDH Antibody (C-term R248), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
GAPDH Antibody - Confocal immunofluorescence of GAPDH Antibody (C-term R248) with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
GAPDH Antibody - Confocal immunofluorescence of GAPDH Antibody (C-term R248) with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
GAPDH Antibody - Western blot of GAPDH Antibody (C-term R248) in A2058, A375, CEM cell line lysates (35 ug/lane). GAPDH (arrow) was detected using the purified antibody.
GAPDH Antibody - Western blot of lysates from HeLa,HUVEC cell line (from left to right),using GAPDH Antibody (C-term R248). Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
GAPDH Antibody - Flow cytometric of HepG2 cells using GAPDH Antibody (C-term R248)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Polyclonal Rabbit anti‑Human GAPDH Antibody (aa233‑259, IHC, IF, WB) LS‑C97485

Polyclonal Rabbit anti‑Human GAPDH Antibody (aa233‑259, IHC, IF, WB) LS‑C97485

Antibody:
GAPDH Rabbit anti-Human Polyclonal (aa233-259) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C97485-400
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
GAPDH Rabbit anti-Human Polyclonal (aa233-259) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
GAPDH antibody LS-C97485 is an unconjugated rabbit polyclonal antibody to human GAPDH (aa233-259). Validated for Flow, IF, IHC and WB.
Target
Human GAPDH
Synonyms
GAPDH | A1 40 kd subunit | Activator 1 40 kd subunit | G3PD | GAPD | G3pdh | Rfc40 | Rf-c 40 kd subunit
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Pig, Chicken (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Epitope
aa233-259
Specificity
This GAPDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 233-259 amino acids from the C-terminal region of human GAPDH.
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GAPDH
P04406 NM_002046 NP_002037.2

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Requested From: United States
Date Requested: 11/23/2024