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Catalog Number Size Price
LS-C331171-20 20 µl $275 
LS-C331171-100 100 µl (3.37 mg/ml) $389 
GAD65 Antibody - Immunohistochemistry of paraffin-embedded rat brain using GAD2 Antibodyat dilution of 1:100 (40x lens).
GAD65 Antibody - Immunofluorescence analysis of HeLa cells using GAD2 antibody. Blue: DAPI for nuclear staining.
GAD65 Antibody - Western blot analysis of extracts of various cell lines, using GAD2 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
GAD65 Antibody - Western blot analysis of extracts of various cell lines, using GAD2 antibody.
GAD65 Antibody - Immunohistochemistry of paraffin-embedded rat brain using GAD2 Antibodyat dilution of 1:100 (40x lens).
GAD65 Antibody - Immunofluorescence analysis of HeLa cells using GAD2 antibody. Blue: DAPI for nuclear staining.
GAD65 Antibody - Western blot analysis of extracts of various cell lines, using GAD2 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
GAD65 Antibody - Western blot analysis of extracts of various cell lines, using GAD2 antibody.
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Polyclonal Rabbit anti‑Human GAD65 Antibody (IHC, IF, WB) LS‑C331171

Polyclonal Rabbit anti‑Human GAD65 Antibody (IHC, IF, WB) LS‑C331171

Antibody:
GAD65 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C331171-20
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
GAD65 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
GAD65 antibody LS-C331171 is an unconjugated rabbit polyclonal antibody to GAD65 from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB.
Target
Human GAD65
Synonyms
GAD2 | GAD-65 | Glutamate decarboxylase 2 | GAD65
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 401-585 of human GAD2 (NP_001127838.1). PLQCSALLVREEGLMQNCNQMHASYLFQQDKHYDLSYDTGDKALQCGRHVDVFKLWLMWRAKGTTGFEAHVDKCLELAEYLYNIIKNREGYEMVFDGKPQHTNVCFWYIPPSLRTLEDNEERMSRLSKVAPVIKARMMEYGTTMVSYQPLGDKVNFFRMVISNPAATHQDIDFLIEEIERLGQDL
Specificity
Human GAD65
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:10 - 1:100)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 65kDa, while the observed MW by Western blot was 70kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GAD65
Q05329 NM_000818 NP_000809.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/12/2024