Target
Mouse FZD2 / Frizzled 2
Synonyms
FZD2 | Dfrizzled2 | Fz2 | Frizzled family receptor 2 | Frizzled-2 | HFz2 | Frizzled homolog 2 | Fz-2 | FzE2 | Mfz10 | Rfz2 | Frizzled 2
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
NS0-derived rmFrizzled-2 extracellular domain.
Epitope
Extracellular Domain
Specificity
Recognizes mouse Frizzled-2. No cross-reactivity was observed with rmFrizzled-1, -3, -4, -6, -7, -8 or rhFrizzled-5.
Applications
- Western blot (1 - 2 µg/ml)
- ELISA
Usage
Suitable for use in Western Blot and Direct ELISA. Western Blot: Use at 1-2 ug/ml with the appropriate secondary reagents to detect mouse Frizzled-2. Using a colorimetric detection system, the detection limit for rmFrizzled-2 is ~25 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively. Direct ELISA: Use at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect mouse Frizzled-2. The detection limit for rmFrizzled-2 is ~5 ng/well.
Presentation
PBS, pH 7.2, 5% Trehalose
Reconstitution
Reconstitute in sterile PBS.
Storage
Lyophilized and resuspended forms: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee