Target
Mouse FZD1 / Frizzled 1
Synonyms
FZD1 | Chfz-1 | Fz1 | Frizzled 1 | Fz receptor 1 | HFz1 | Frizzled homolog 1 | Fz-1 | FzE1 | Rfz1 | Xfrizzled-1 | Wnt receptor | Dfrizzled-1 | Frizzled family receptor 1 | Frizzled-1
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
NS0-derived rmFrizzled-1 extracellular domain.
Epitope
Extracellular Domain
Specificity
Recognizes mouse Frizzled-1. Shows no cross-reactivity with rmFrizzled-2, -3, -4, -6, -7 or -8.
Applications
- Western blot (1 - 2 µg/ml)
- ELISA
Usage
Suitable for use in Western Blot and Direct ELISA. Western Blot: Use at 1-2 ug/ml with the appropriate secondary reagents to detect mouse Frizzled-1. Using a colorimetric detection system, the detection limit for rmFrizzled-1 is ~5 ng/lane and 1 ng/lane under non-reducing and reducing conditions, respectively. Direct ELISA: Used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect mouse Frizzled-1. The detection limit for rmFrizzled-1 is ~5 ng/well.
Presentation
PBS, pH 7.2, 5% Trehalose
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee