Target
Mouse FZD1 / Frizzled 1
Synonyms
FZD1 | Chfz-1 | Fz1 | Frizzled 1 | Fz receptor 1 | HFz1 | Frizzled homolog 1 | Fz-1 | FzE1 | Rfz1 | Xfrizzled-1 | Wnt receptor | Dfrizzled-1 | Frizzled family receptor 1 | Frizzled-1
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Purification
Immunoaffinity purified
Immunogen
NS0-derived recombinant, murine, Frizzled-1 extracellular Cysteine-rich Domain (CRD).
Specificity
Recognizes mouse Frizzled-1.
Applications
- IHC
- Western blot (0.1 - 0.2 µg/ml)
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Usage
Suitable for use in Western Blot and Immunohistochemistry. Western Blot: Use at 0.1-0.2 ug/ml with the appropriate secondary reagents to detect mouse Frizzled-1. The detection limit for rmFrizzled-1 is ~2 ng/lane under non-reducing and reducing. conditions. In this format, this antibody shows approximately 5% cross-reactivity with rmFrizzled-3, rmFrizzled-4, rmFrizzled-7, rmFrizzled-8 and less than 1% cross-reactivity with rmFrizzled-6. Immunohistochemistry: Detects Frizzled-1 in cells and tissues at 15 ug/ml. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from PBS, pH 7.3, 0.1% BSA.
Reconstitution
Sterile Tris-buffered saline pH 7.3, containing 0.1% BSA
Storage
Lyophilized and resuspended forms: store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee