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Catalog Number Size Price
LS-C358987-30 30 µl (1 mg/ml) $255 
LS-C358987-100 100 µl (1 mg/ml) $299 
LS-C358987-200 200 µl (1 mg/ml) $351 
FFAR2 / GPR43 Antibody - Immunohistochemical analysis of GPR43 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
FFAR2 / GPR43 Antibody - Immunofluorescent analysis of GPR43 staining in THP1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ??C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
FFAR2 / GPR43 Antibody - Western blot analysis of GPR43 expression in THP1 (A) whole cell lysates.
FFAR2 / GPR43 Antibody - Immunohistochemical analysis of GPR43 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
FFAR2 / GPR43 Antibody - Immunofluorescent analysis of GPR43 staining in THP1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ??C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
FFAR2 / GPR43 Antibody - Western blot analysis of GPR43 expression in THP1 (A) whole cell lysates.
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Polyclonal Rabbit anti‑Human FFAR2 / GPR43 Antibody (C‑Terminus, IHC, IF, WB) LS‑C358987

Polyclonal Rabbit anti‑Human FFAR2 / GPR43 Antibody (C‑Terminus, IHC, IF, WB) LS‑C358987

Antibody:
FFAR2 / GPR43 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$255
LS-C358987-30
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
FFAR2 / GPR43 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
GPR43 antibody LS-C358987 is an unconjugated rabbit polyclonal antibody to human GPR43 (FFAR2) (C-Terminus). Validated for ICC, IF, IHC and WB.
Target
Human FFAR2 / GPR43
Synonyms
FFAR2 | FFA2R | Free fatty acid receptor 2 | G protein-coupled receptor 43 | G-protein coupled receptor 43 | LSSIG | GPR43 | Gpcr43
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human GPR43.
Epitope
C-Terminus
Specificity
Recognizes endogenous levels of GPR43 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FFAR2 / GPR43
O15552 NM_005306 NP_005297.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024