Human FEN1

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Purified

Unmodified

Synthetic peptide corresponding to a region within the N-Terminus domain of human FEN1 (locus link ID 2237).

N-Terminus

Recognizes human Flap endonuclease 1 also known as Flap structure-specific endonuclease, FEN1, Maturation factor 1 or DNase IV. FEN1 is a 380 amino acid ~45kD member of the FEN1 sub family of XPG/RAD2 endonucleases which has an important role in lagging strand DNA synthesis and base excision repair. It is hypothesised that FEN1 may play a role in the inhibition of triplet repeat expansion. Two isoforms of FEN1 are generated by alternative initiation, isoform 1 against whichis generated and a truncated isoform FENMIT, lacking the N-terminal region and having a mitochondrial distribution, FENMIT also lacks DNAse activity. Initial results suggest thatdoes not recognize the truncated form of human FEN1. Fen1 is expressed in the nucleus of a wide variety of organ structures notably in those of the hematopoietic system including spleen, lymph nodes and spleen. Recognizes a band of ~45kD in whole cell lysates of HeLa and 293T by western blotting and has successfully been used for immunoprecipitation of FEN1 from HeLa cell lysates.

• Western blot (1:5000 - 1:30000)
• Immunoprecipitation

Western Blot: LS-C43020 detects a band of approximately 42kD in Hela cell lysates.

Tris-Citrate/PBS, 0.09% Sodium Azide

Store at 4°C. Do not freeze. Store undiluted. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P39748 NCBI: NM_004111 NP_004102.1