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Antibodies > FEN1 > LS-C352190
 
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Catalog Number Size Price
LS-C352190-100 100 µl (1 mg/ml) $299 
FEN1 Antibody - Immunohistochemical analysis of FEN1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
FEN1 Antibody - Immunofluorescent analysis of FEN1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
FEN1 Antibody - Western blot analysis of FEN1 expression in A431 (A); MCF7 (B); U937 (C); NIH3T3 (D); PC12 (E) whole cell lysates.
FEN1 Antibody - Immunohistochemical analysis of FEN1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
FEN1 Antibody - Immunofluorescent analysis of FEN1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
FEN1 Antibody - Western blot analysis of FEN1 expression in A431 (A); MCF7 (B); U937 (C); NIH3T3 (D); PC12 (E) whole cell lysates.
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Polyclonal Rabbit anti‑Human FEN1 Antibody (Internal, IHC, IF, WB) LS‑C352190

Polyclonal Rabbit anti‑Human FEN1 Antibody (Internal, IHC, IF, WB) LS‑C352190

Submit Review LSBio Guarantee
Antibody:
FEN1 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human, Monkey, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C352190-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
FEN1 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human, Monkey, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
FEN1 antibody LS-C352190 is an unconjugated rabbit polyclonal antibody to FEN1 (Internal) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC, IP and WB.
Target
Human FEN1
Synonyms
FEN1 | DNase IV | FEN-1 | HFEN-1 | Flap endonuclease 1 | MF1 | RAD2 | Maturation factor 1 | Maturation factor-1
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human FEN1.
Epitope
Internal
Specificity
Recognizes endogenous levels of FEN1 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:10 - 1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FEN1
P39748 NM_004111 NP_004102.1

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Featured Products

FEN1 Antibody - Anti-FEN1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ FEN1 Antibody (aa1‑380, clone 4E7) LS‑B1694
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
FEN1 Antibody - Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using FEN1 Antibody. The picture on the right is blocked with the synthesized peptide.
FEN1 Antibody (aa86‑135) LS‑C118882
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
FEN1 Antibody - Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 1.25 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
FEN1 Antibody (aa301‑350) LS‑C80825
Species: Human
Applications: Western blot
Product
Human FEN1 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G75948
Source: Human
Tag: Myc-DDK (Flag)
See all FEN1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/26/2024
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