Target
Human FcERI / Fc Epsilon RI
Synonyms
FCER1A | Alpha polypeptide | FCE1A | FcERI | Fc Epsilon RI | Fc epsilon RI alpha-chain | Fc-epsilon RI-alpha | IgE Fc receptor subunit alpha
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-TGKVWQLDYESEP, from the internal region of the protein sequence according to NP_001992.1.
Applications
- Western blot (0.3 - 1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 50kDa band observed in Human Pripheral Blood Mononucleocytes lysates (calculated MW of 29.6kDa according to NP_001992.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Ganguly et al, J Biol Chem. 2007 Nov 9;282(45):32758-64.PMID: 17728245). Recommended concentration: 0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FcERI / Fc Epsilon RI